T-lymphocytes in experimental Leishmania amazonensis infection: comparison between immunized and naive BALB/c mice.
T-lymphocytes in experimental Leishmania amazonensis infection: comparison between immunized and naive BALB/c mice.
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实验性亚马逊利什曼原虫感染中的 T 淋巴细胞：免疫 BALB/c 小鼠与幼稚 BALB/c 小鼠之间的比较。
DOI：
10.1007/bf00936175
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发表时间：
1992
影响因子：
2
通讯作者：
Barral-Netto,M
中科院分区：
文献类型：
--
作者：
Pompeu,M;Freitas,AL;dosReis,GA;Barral-Netto,M
为了鉴定和鉴定血吸虫血吸虫半胱氨酸蛋白酶，对冻干成虫进行匀浆和酶的分离纯化。从酸性缓冲液中制备的提取物中，直接或间接鉴定了3种可能的半胱氨酸蛋白酶。第一个蛋白(ShCP1)用放射性碘标记的抑制剂Z-Tyr-Ala-CHN2标记，确定为35 kDa的蛋白。然而，它不能通过银染、氨基酸测序或针对曼氏血吸虫类似分子的单抗来检测。第二种半胱氨酸蛋白酶ShCP2通过凝胶过滤和透析法纯化。这个32 kDa的分子是硫醇依赖的，并被Z-Tyr-Ala-CHN_2标记。ShCP2的氨基端氨基酸序列与曼氏葡萄球菌组织蛋白B(SmCP2)以及哺乳动物半胱氨酸蛋白酶有显著的相似性(达77%)。ShCP1和ShCP2均与曼氏血吸虫多克隆抗体反应，提示存在共同的抗原表位。根据其与曼氏链霉菌28 kDa半胱氨酸蛋白酶的相似性，第三种活性蛋白ShCP3可能是一种独特的水解酶。这一初步研究表明，血瘤链霉菌中的半胱氨酸蛋白酶的总体特征与曼氏链霉菌非常相似。
To identify and characterize cysteine proteinases from Schistosoma haematobium, lyophilized adult worms were homogenized, and enzymes were isolated and purified. From extracts prepared in acidic buffer, 3 putative cysteine proteinases were identified either directly or indirectly. The first proteinase (ShCP1) was identified by labeling with a radioiodinated inhibitor, Z-Tyr-Ala-CHN2, as a 35-kDa protein. However, it could not be detected by silver staining, amino acid sequencing, or by a monoclonal antibody specific for a similar molecule from Schistosoma mansoni. A second cysteine proteinase, ShCP2, was purified by gel filtration and dialysis. This 32-kDa molecule was thiol-dependent and was labeled with Z-Tyr-Ala-CHN2. The amino terminal amino acid sequence of ShCP2 showed remarkable similarity (up to 77%) to that of S. mansoni cathepsin B (SmCP2) as well as to mammalian cysteine proteinases. Both ShCP1 and ShCP2 reacted with polyclonal antibodies against S. mansoni, suggesting the existence of shared antigenic epitopes. A third activity, ShCP3, was identified as possibly a distinct proteinase based on its similarities to a 28-kDa cysteine proteinase from S. mansoni. This preliminary investigation demonstrates that the overall profile of cysteine proteinases in S. haematobium is very similar to that of S. mansoni.