Melatonin and type 2 diabetes -: a possible link?
Melatonin and type 2 diabetes -: a possible link?
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DOI：
10.1111/j.1600-079x.2007.00426.x
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发表时间：
2007-04-01
影响因子：
10.3
通讯作者：
Muehlbauer, Eckhard
中科院分区：
文献类型：
--
作者：
Peschke, Elmar;Stumpf, Ina;Muehlbauer, Eckhard
本研究的目的是确定人胰腺组织中褪黑素膜受体的存在和核孤儿受体的mRNA表达，以试图解释2型糖尿病患者和代谢健康患者之间的差异。分子和免疫细胞化学研究证实，褪黑素膜受体MT1和MT2在人胰腺组织中存在，特别是在朗格汉斯的胰岛中也存在。确定mRNA表达比率的计算模型以及免疫反应的主观分析结果显示，MT1受体的表达高于MT2的表达。2型糖尿病患者褪黑素受体的mRNA转录水平明显高于对照组。在免疫细胞化学研究中也观察到2型糖尿病患者受体表达上调。此外，在人胰腺组织和胰岛中还检测到核孤儿受体RORα、RZRβ、ROR伽马和混响α的转录本。与膜褪黑素受体相关的数据表明，在2型糖尿病患者中，RORα、RZRβ和ROR Gamma的mRNA表达水平增加。因此，我们的研究结果表明，在人胰腺组织中存在褪黑素膜受体MT1和MT2以及核孤儿受体的mRNA表达，并且在2型糖尿病患者中表达上调。
The aim of the present study was to determine the existence of melatonin membrane receptors and to examine the mRNA expression of nuclear orphan receptors in human pancreatic tissue, in an effort to explain differences between type 2 diabetic and metabolically healthy patients. Molecular and immunocytochemical investigations established the presence of the melatonin membrane receptors MT1 and MT2 in human pancreatic tissue and, notably, also in the islets of Langerhans. Results of a calculation model to determine mRNA expression ratios, as well as subjective analysis of immunoreactions, showed elevated MT1 receptor expression in comparison with MT2 expression. mRNA transcript levels of melatonin receptors appeared to be significantly higher in type 2 diabetic patients than in a control group. An upregulation of receptor expression in type 2 diabetic patients was also observed in immunocytochemical investigations. In addition, transcripts of the nuclear orphan receptors ROR alpha, RZR beta, ROR gamma and RevErb alpha were detected in human pancreatic tissue and islets. In correlation with membrane melatonin receptors, data indicate increased mRNA expression levels of ROR alpha, RZR beta, and ROR gamma in type 2 diabetic patients. Thus, our data demonstrate the existence of the melatonin membrane receptors MT1 and MT2 as well as mRNA expression of nuclear orphan receptors in human pancreatic tissue, with upregulated expression levels in type 2 diabetic patients.