IMPEDANCE BASED NANOTOXICITY ASSESSMENT OF GRAPHENE NANOMATERIALS AT THE CELLULAR AND TISSUE LEVEL
IMPEDANCE BASED NANOTOXICITY ASSESSMENT OF GRAPHENE NANOMATERIALS AT THE CELLULAR AND TISSUE LEVEL
复制标题
DOI：
10.1080/00032719.2011.633184
复制
发表时间：
2012-01-01
影响因子：
2
通讯作者：
Li, Chen-Zhong
中科院分区：
文献类型：
--
作者：
Hondroulis, Evangelia;Zhang, Zhiqi;Li, Chen-Zhong
过去几年，人们对石墨烯在生物医学应用方面的兴趣大幅增长，从而产生了对生物相容性测试的需求。使用石墨烯的生物医学工程应用，如生物传感装置、微生物检测、疾病诊断和药物输送系统，正在迅速发展，但可能忽略了任何可能的危害，因为石墨烯纳米材料与生物材料的相互作用可能不同于碳纳米管和富勒烯等其他石墨材料。由于石墨烯的潜在应用是药物输送，因此通过测量跨内皮电阻（TEER），针对血脑屏障（BBB）的体外模型测试了石墨烯的毒性。还利用电阻抗传感方面的新方法，通过测量阻抗响应，实时动力学分析石墨烯纳米材料对 BBB 模型的各个成分、大鼠星形胶质细胞 (CRL-2006) 和小鼠内皮细胞 (CRL-2583) 的细胞毒性。石墨烯对两种单独的细胞类型几乎没有或没有毒性，因为电阻测量结果与对照相似，此外，石墨烯没有破坏 BBB 模型的完整性，显示出石墨烯的生物相容性以及这些新型纳米材料在生物医学应用中的广泛潜力。
The interest in graphene for biomedical applications has grown substantially in the past few years creating a need for biocompatibility testing. Biomedical engineering applications using graphene such as biosensing devices, microbial detection, disease diagnosis, and drug delivery systems are progressing rapidly, perhaps overlooking any possible hazards as graphene nanomaterials may interact with biological materials differently than other graphitic materials such as carbon nanotubes and fullerenes. As a potential application for graphene is drug delivery, the toxicity of graphene was tested against an in vitro model of the blood brain barrier (BBB) by measuring trans-endothelial-electrical resistance (TEER). A new approach in terms of electrical impedance sensing was also utilized to kinetically analyze the cytotoxicity of graphene nanomaterials towards the BBB model's individual components, rat astrocytes (CRL-2006) and mouse endothelial cells (CRL-2583), in real time by measuring the impedimetric response. Graphene showed little or no toxicity toward both individual cell types as the resistance measurements were similar to those of the control and further, graphene did not interrupt the integrity of the BBB model as a whole showing the biocompatibility of graphene and the broad potential of using these new nanomaterials for biomedical applications.