The degradation of polygalacturonic acid by rumen ciliate protozoa.
The degradation of polygalacturonic acid by rumen ciliate protozoa.
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瘤胃纤毛虫对聚半乳糖醛酸的降解。
DOI：
10.1099/00221287-120-2-295
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
S. Beahon
中科院分区：
文献类型：
--
作者：
G. Coleman;D. C. Sandford;S. Beahon
9种瘤胃纤毛虫原虫和两种混合原虫制剂的无细胞提取物的解聚酶活性，在体内和体外生长，对聚半乳糖醛酸进行了检查。最高的活性被发现与Eremoplastron bovis和Ostracodinium obtusum bilobum，而有刺或无刺形式的尾内甲藻和Polyplastron multisticulatum很少。基于粘度的快速下降、EDTA的抑制和u. v. -在吸收材料中，来自所有活性物种的酶被指定为内切果胶裂解酶（EC 4.2.2.2），尽管可能存在一些多聚半乳糖醛酸酶。果胶和聚半乳糖醛酸都不支持任何测试的原生动物物种的生存或生长。
The depolymerase activity of cell-free extracts of nine species of rumen ciliate protozoa and two mixed protozoal preparations, grown in vivo and in vitro, towards polygalacturonic acid was examined. The highest activity was found with Eremoplastron bovis and Ostracodinium obtusum bilobum while there was none in the spined or spineless forms of Entodinium caudatum and little in Polyplastron multivesticulatum. On the basis of the rapid drop in viscosity, inhibition by EDTA and the production of u.v.-absorbing material, the enzymes from all active species were designated as endopectate lyases (EC4.2.2.2) although some polygalacturonase may be present. Neither pectin nor polygalacturonic acid supported the survival or growth of any of the protozoal species tested.