CHARACTERIZATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME (SIRS) VIRUS (ISOLATE ATCC VR-2332)
CHARACTERIZATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME (SIRS) VIRUS (ISOLATE ATCC VR-2332)
复制标题
DOI：
10.1177/104063879200400202
复制
发表时间：
1992-04-01
影响因子：
1.5
通讯作者：
CHLADEK, D
中科院分区：
文献类型：
--
作者：
BENFIELD, DA;NELSON, E;CHLADEK, D
本文报道了一株猪不孕与呼吸综合征（SIRS）病毒分离株（ATCCVR-2332）的特性。所有试验均使用商业细胞系（CL 2621）进行病毒增殖。实验室研究表明，该分离株是一种苛养、非血凝、有包膜RNA病毒。氯化铯纯化的病毒粒子通过电子显微镜观察为平均直径为62 nm（范围：48-83 nm）的球形颗粒和被包膜包围的25-30 nm核心。免疫荧光显示，病毒复制仅限于细胞质内。该病毒与几种常见猪病毒的抗血清或甲病毒属、风疹病毒属、瘟病毒属和披膜病毒科未分组乳酸脱氢酶病毒属中已知病毒的抗血清不发生血清学反应。然而，恢复期母猪血清和兔超免疫血清中和SIRS病毒的滴度分别为1：256和1：512。该病毒在4 ℃和-70 ℃下稳定，但在37 ℃和56 ℃下不稳定。该SIRS病毒分离株的特性类似于披膜病毒科的特性，但与所描述的属不匹配。
The characterization of an isolate of swine infertility and respiratory syndrome (SIRS) virus (ATCC VR-2332) is reported. A commercial cell line (CL2621) was used for the propagation of the virus for all assays. Laboratory studies indicate that this isolate is a fastidious, nonhemagglutinating, enveloped RNA virus. Cesium chloride-purified virions visualized by electron microscopy were spherical particles with an average diameter of 62 nm (range: 48-83 nm) and a 25-30 nm core surrounded by an envelope. Virus replication was restricted to the cytoplasm, as demonstrated by immunofluorescence. The virus did not react serologically with antisera to several common porcine viruses or with antisera to known viruses in the alphavirus, rubivirus, pestivirus, and ungrouped lactic dehydrogenase virus genera of the Togaviridae. However, convalescent sow sera and rabbit hyperimmune sera neutralized the SIRS virus at titers of 1:256 and 1:512, respectively. The virus was stable at 4 and -70 C, but was labile at 37 and 56 C. The properties of this isolate of SIRS virus resemble those of the family Togaviridae but do not match the described genera.