p59fyn tyrosine kinase regulates p56lck tyrosine kinase activity and early TCR-mediated signaling.
p59fyn tyrosine kinase regulates p56lck tyrosine kinase activity and early TCR-mediated signaling.
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p59fyn 酪氨酸激酶调节 p56lck 酪氨酸激酶活性和早期 TCR 介导的信号传导。
DOI：
10.1093/intimm/6.10.1621
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发表时间：
1994
影响因子：
4.4
通讯作者：
Bothwell,AL
中科院分区：
文献类型：
--
作者：
Lee,SK;Shaw,A;Maher,SE;Bothwell,AL
为了研究 p59fynin T 细胞激活的作用，我们使用反义 RNA 抑制 T 细胞克隆中的 p59fyn 表达。 p59fyn 水平降低的转染子对抗原、Con A + 重组 IL-1 和与抗 TCR mAb 交联的反应功能受损。大多数细胞内底物上酪氨酸磷酸化的诱导大大减少。我们还注意到，即使 Ick 蛋白的量与亲本 D10 细胞中存在的量相当，Ick 激酶活性也大大降低。我们的结果表明蛋白酪氨酸激酶 p59fy 在 TCR 介导的信号传导中至关重要，并且还表明 p59fyn 可能调节 p56fyn酪氨酸激酶活性。
To study the role of p59fynin T cell activation, we used antisense RNA to inhibit p59fynexpression in a T cell clone. Transfectants with reduced levels of p59fynwere functionally impaired in their responses to antigen, Con A + recombinant IL-1 and cross-linking with anti-TCR mAb. Induction of tyrosine phosphorylation on most intracellular substrates was greatly reduced. We also noted that the Ick kinase activity was greatly reduced even though the amount of Ick protein was equivalent to that present in parental D10 cells. Our results suggest that the protein tyrosine kinase p59fynis critical in TCR-mediated signaling and also suggests that p59fynmay regulate p56fyntyrosine kinase activity.