Involvement of Free Calcium in Action of Cryptogein, a Proteinaceous Elicitor of Hypersensitive Reaction in Tobacco Cells-猫眼课题宝

游离钙参与烟草细胞过敏反应的蛋白质引发剂隐黄素的作用

DOI：

10.1104/pp.109.3.1025

发表时间：

1995

期刊：

The Plant journal : for cell and molecular biology

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

E. Tavernier;D. Wendehenne;J. Blein;A. Pugin

用隐地菌素（Phytophthora cryptogea的一种蛋白质激发子）处理悬浮培养的烟草细胞，在最初的几分钟内诱导了一个很大的钙内流刺激。Ca 2+内流对于隐地蛋白诱导的反应的起始是必不可少的，因为乙二醇-双（β-氨基-乙基醚）-N，N[prime]-四乙酸或La 3+，其阻断Ca 2+入口，抑制隐地蛋白诱导的反应，如细胞外碱化、活性氧和植物防御素的产生。此外，一旦启动，这些反应需要在第1 h内持续的Ca 2+内流。钙离子载体（A23187）能够触发细胞外碱化，但不形成活性氧和植物抗毒素，即使在存在的隐地蛋白。Staurosporine是一种蛋白激酶抑制剂，最近报道可抑制隐地蛋白诱导的反应（M.- P. Viard，F. Martin，A. Pugin，P. Ricci，J. P. Blein [1994] Plant Physiol 104：1245-1249）以剂量依赖性方式抑制由隐地蛋白诱导的Ca 2+内流。这些结果表明，蛋白磷酸化后的Ca ~（2+）内流可能参与了cryptogein信号转导的初始步骤。

Treatment of suspension-cultured tobacco (Nicotiana tabacum var Xanthi) cells with cryptogein, a proteinaceous elicitor from Phytophthora cryptogea, induced a great stimulation of Ca2+ influx within the first minutes. Ca2+ influx is essential for the initiation of cryptogein-induced responses, since ethyleneglycol-bis([beta]-amino-ethyl ether)-N,N[prime]-tetraacetic acid or La3+, which block Ca2+ entrance, suppress cryptogein-induced responses such as extracellular alkalinization, active oxygen species, and phytoalexin production. Moreover, once initiated, these responses require sustained Ca2+ influx within the 1st h. A Ca2+ ionophore (A23187) was able to trigger an extracellular alkalinization but not the formation of active oxygen species and phytoalexins, even in the presence of cryptogein. Staurosporine, a protein kinase inhibitor that was recently reported to suppress cryptogein-induced responses (M.-P. Viard, F. Martin, A. Pugin, P. Ricci, J.-P. Blein [1994] Plant Physiol 104: 1245–1249), inhibited Ca2+ influx induced by cryptogein in a dose-dependent manner. These results suggest that protein phosphorylation followed by Ca2+ influx might be involved in the initial steps of cryptogein signal transduction.

Cytokinin stimulates dihydropyridine-sensitive calcium uptake in moss protoplasts.