Dynamics of Cutibacterium repopulation onto the skin surface of the shoulder after chlorhexidine application.
Dynamics of Cutibacterium repopulation onto the skin surface of the shoulder after chlorhexidine application.
复制标题
使用氯己定后，皮肤杆菌在肩部皮肤表面重新繁殖的动态。
DOI：
10.1007/s00264-023-05786-x
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发表时间：
2023
影响因子：
2.7
通讯作者：
Long,DustinR
中科院分区：
文献类型：
--
作者：
Hsu,JasonE;Whitson,AnastasiaJ;VanDyke,Rufus;Wu,JohnC;Matsen3rd,FrederickA;Long,DustinR
PurposeThe的目的是本研究的特点的时间动态OCutibacteriumrepopulation的皮肤表面应用洗必泰shoulder. Methods 10肩5名男性受试者。在使用2%葡萄糖酸氯己定和70%异丙醇准备皮肤之前（0分钟）以及之后3、30、60、120和240分钟采集皮肤拭子。半定量的细菌负荷测定为每个timepoint.ResultsFrom零分钟（预处理）到三分钟，洗必泰，异丙醇减少了皮肤细菌负荷在八个十个肩膀。在这8个肩关节中，4个肩关节（50%）在30分钟时生长，7个肩关节（88%）在60分钟时生长，所有8个肩关节（100%）在240分钟时生长。洗必泰应用程序后的三分钟相比，细菌负荷显着增加了60分钟，但仍显着低于pre-prep细菌负荷（0分钟）。Conclusionafter标准的外科手术皮肤准备洗必泰-异丙醇，表面的肩膀是repopulated与Cutibacterium在一个小时内，大概是从水库中的皮脂腺局部防腐剂不渗透。由于这些真皮腺体是通过肩关节成形术的皮肤切口横断的，因此本研究表明，尽管使用洗必泰进行了皮肤准备，但它们可能是手术过程中伤口污染的来源。
PurposeThe objective of this study was to characterize the temporal dynamics ofCutibacteriumrepopulation of the skin surface after application of chlorhexidine to the shoulder.MethodsTen shoulders in five male subjects were used. A skin swab was taken prior to (0 minutes) and then at three, 30, 60, 120, and 240 minutes after skin preparation with 2% chlorhexidine gluconate and 70% isopropyl alcohol. Semi-quantitative bacterial load was measured for each timepoint.ResultsFrom zero minutes (pre-treatment) to three minutes, chlorhexidine-isopropyl alcohol reduced the skin bacterial load in eight out of ten shoulders. Of these eight shoulders, four (50%) had growth by 30 minutes, seven (88%) had growth by 60 minutes, and all eight (100%) had growth by 240 minutes. Compared to the three minutes after chlorhexidine application, bacterial load had significantly increased by 60 minutes but were still significantly lower than the pre-prep bacterial load (0 minutes).ConclusionFollowing standard surgical skin preparation with chlorhexidine-isopropyl alcohol, the surface of the shoulder is repopulated withCutibacteriumwithin one hour, presumably from reservoirs in sebaceous glands not penetrated by topical antiseptic agents. Since these dermal glands are transected by skin incision for shoulder arthroplasty, this study suggests that they may be sources of wound contamination during surgery in spite of skin preparation with chlorhexidine.