Evidence for a membrane-bound fraction of chick intestinal calcium-binding protein.
Evidence for a membrane-bound fraction of chick intestinal calcium-binding protein.
复制标题
鸡肠钙结合蛋白膜结合部分的证据。
DOI：
10.1016/0304-4165(78)90442-7
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
R. Wasserman
中科院分区：
文献类型：
--
作者：
J. Feher;R. Wasserman
将富含维生素 D 的雏鸡的肠粘膜匀浆并高速离心后，大部分维生素 D 诱导的钙结合蛋白存在于上清液部分中。然而，反复洗涤后的离心液含有大量可被 Triton X-100 溶解的结合钙结合蛋白。根据凝胶过滤色谱法测定的免疫学特性、电泳迁移率和分子大小的标准，结合的钙结合蛋白与可溶性钙结合蛋白相同。结合的钙结合蛋白仅通过超声处理、渗透压休克或核糖核酸酶处理部分释放。结合的和可溶性的钙结合蛋白不存在于佝偻病鸡肠中。在均质化之前向佝偻粘膜中添加钙结合蛋白不会产生 Triton X-100 可溶形式，这表明维生素 D 充足的肠道中结合的钙结合蛋白不是由于可溶性钙结合蛋白的囊泡捕获而导致的。总体证据表明，部分肠钙结合蛋白是膜结合的。
After homogenization of intestinal mucosa from vitamin D-replete chicks and high speed centrifugation, the major proportion of the vitamin D-induced calcium-binding protein is present in the supernatant fraction. However, the centrifugate, after repeated washing, contains significant amounts of bound calcium-binding protein that can be solubilized by Triton X-100. The bound calcium-binding protein is identical to soluble calcium-binding protein by the criteria of immunological identity, electrophoretic mobility, and molecular size, as determined by gel filtration chromatography. The bound calcium-binding protein is only partially released by sonication, osmotic shock or by ribonuclease treatment Bound and soluble calcium-binding protein are not present in rachitic chick intestine. The addition of calcium-binding protein to rachitic mucosa prior to homogenization does not yield a Triton X-100 solubilizable form, indicating that bound calcium-binding protein in vitamin D-replete intestine is not due to adsorption of vesicular entrapmetn of soluble calcium-binding protein. The overall evidence suggests that part of the intestinal calcium-binding protein is membrane-bound.