Quantitation of estrogen receptors and relaxin binding in human anterior cruciate ligament fibroblasts
Quantitation of estrogen receptors and relaxin binding in human anterior cruciate ligament fibroblasts
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DOI：
10.1290/0512089.1
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发表时间：
2006-07-01
影响因子：
2.1
通讯作者：
Hannafin, Jo A.
中科院分区：
文献类型：
--
作者：
Faryniarz, Deborah A.;Bhargava, Madhu;Hannafin, Jo A.
大学生女性前交叉韧带损伤发生率明显高于男性，可能是由于前交叉韧带相对松弛所致。雌激素和松弛素活性的差异，与怀孕时的情况相似，可能是造成这种情况的原因。我们用流式细胞术对人类前交叉韧带细胞中的雌激素受体和松弛素受体进行了定量，并比较了这些受体在男性和女性中的存在。前交叉韧带残端取自7名男性和8名女性急性前交叉韧带损伤患者。将组织置于M199细胞培养液中。获得生长培养物，并使用第2代细胞进行所有研究。用流式细胞仪进行雌激素受体测定。使用I-125标记的松弛素在来自5名女性和男性患者的前交叉韧带细胞中进行松弛素结合。流式细胞术在4%至10%的前交叉韧带细胞中检测到雌激素受体。表达雌激素受体的细胞的平均荧光大约是对照组的两倍，男性和女性之间没有显著差异。松弛素研究显示，I-125-松弛素标记的前交叉韧带细胞有低水平的结合。松弛素结合存在于五分之四的女性前交叉韧带细胞中，而五分之一的男性前交叉韧带细胞中存在松弛素结合。
The significantly higher incidence of anterior cruciate ligament (ACL) injuries in collegiate women compared with men may result from relative ligament laxity. Differences in estrogen and relaxin activity, similar to that seen in pregnancy, may account for this. We quantified estrogen receptors by flow cytometry and relaxin receptors by radioligand binding assay in human ACL cells and compared the presence of these receptors in males and females. ACL stumps were harvested from seven males and eight females with acute ACL injuries. The tissue was placed in M199 cell culture medium. Outgrowth cultures were obtained, and passage 2 cells were used for all studies. Estrogen receptor determination was performed using flow cytometry. Relaxin binding was performed in ACL cells derived from five female and male patients using I-125-labeled relaxin. Estrogen receptors were identified by flow eytometry in 4 to 10% of ACL cells. Mean fluorescence of cells expressing estrogen receptors was approximately twice that of controls, with no significant differences between males and females. Relaxin studies showed low-level binding of I-125,-relaxin-labeled ACL cells. Relaxin binding was present in four out of five female ACL cells versus one out of five male ACL cells.