Distribution visualization of the chlorinated disinfection byproduct of diazepam in zebrafish with desorption electrospray ionization mass spectrometry imaging.
Distribution visualization of the chlorinated disinfection byproduct of diazepam in zebrafish with desorption electrospray ionization mass spectrometry imaging.
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DOI：
10.1016/j.talanta.2021.122919
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发表时间：
2022-01
期刊：
影响因子：
6.1
通讯作者：
Xiaole Zhao;Xiaoyong Huang;Xin Zhang;Jiachen Shi;Xiaofei Jia;Kui Zhu;B. Shao
中科院分区：
文献类型：
--
作者：
Xiaole Zhao;Xiaoyong Huang;Xin Zhang;Jiachen Shi;Xiaofei Jia;Kui Zhu;B. Shao
地西泮（DZP）是一种常规处方，用于治疗大量患有焦虑症的人群。然而，由于DZP及其氯化消毒副产物2-甲氨基-5-氯二苯甲酮（MACB）的过度使用和滥用，造成了环境污染，在北京市饮用水中普遍存在。然而，关于MACB的代谢动力学的信息知之甚少。本文建立了解吸电喷雾电离质谱成像方法，对MACB在斑马鱼体内的分布和代谢进行了可视化和定量的研究。结果表明，MACB特异性地聚集在脊髓，特别是在雌性斑马鱼。同时，MACB的积聚可通过血脑屏障（BBB），诱导小胶质细胞吞噬神经元。因此，应探索有效的干预策略，限制此类物质的释放，消除对人类和生态环境的潜在风险。
Diazepam (DZP) was routinely prescribed to a large population troubled with anxiety disorders. However, due to the overuse and misuse, DZP and its chlorination disinfection byproduct 2-methylamino-5-chlorobenzophenone (MACB) caused environmental pollution and can be detected ubiquitously in drinking water in Beijing, China. However, little information is known about the metabolic dynamics of MACB. Here, we established desorption electrospray ionization mass spectrometry (DESI-MS) imaging method to visually and quantitatively assess the distribution and metabolism of MACB in zebrafish. The results showed that MACB specifically accumulated in spinal cord particularly in female zebrafish. Meanwhile, the accumulation of MACB could pass through the blood-brain barrier (BBB) and induced microglial phagocytosis of neurons. Therefore, the intervention strategies should be explored to restrict the release of such substances, eliminating the potential risks for both human beings and the eco-environment.