Target-Site Resistance Mechanisms to Tribenuron-methyl and Cross-resistance Patterns to ALS-inhibiting Herbicides of Catchweed Bedstraw (Galium aparine) with Different ALS Mutations
Target-Site Resistance Mechanisms to Tribenuron-methyl and Cross-resistance Patterns to ALS-inhibiting Herbicides of Catchweed Bedstraw (Galium aparine) with Different ALS Mutations
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DOI：
10.1017/wsc.2018.70
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发表时间：
2019-03-01
期刊：
影响因子：
2.5
通讯作者：
Yuan, Shuzhong
中科院分区：
文献类型：
--
作者：
Deng, Wei;Di, Yingjie;Yuan, Shuzhong
猪殃殃（Galium aparine L.）是一种严重的双子叶杂草，发生在主要的冬小麦（Triticum aestivum L.）中国的田野自1988年以来，苯磺隆在我国广泛应用于阔叶杂草的防除。然而，过度使用导致G.肝素到苯磺隆。在本研究中，20 G.以山东和河南两省的小麦群体为材料，研究了小麦对苯磺隆的抗性及其靶点抗性机制。在剂量反应实验中，12 G.与敏感种群相比，不同种群对苯磺隆表现出不同的抗性水平（2.92 ~ 842.41倍）。在不同抗性群体中检测到5种不同的乙酰乳酸合成酶（ALS）突变（Pro-197-Leu、Pro-197-Ser、Pro-197-His、Asp-376-Glu和Trp-574-Leu）。在耐药群体（HN 6）中也观察到Pro-197-Ser和Trp-574-Leu突变杂合个体。此外，产生了对于特定ALS突变单独纯合的pHB 4（Pro-197-Ser）、pHB 7（Pro-197-His）、pHB 8（Pro-197-Leu）、pHB 5（Asp-376-Glu）和pHB 3（Trp-574-Leu）亚群，以评估对ALS抑制性除草剂的交叉抗性。pHB 4、pHB 7、pHB 8、pHB 5和pHB 3亚群均对磺酰脲类、嘧磺隆、三唑嘧啶、嘧磺草胺、磺酰氨基羰基三唑啉酮、氟卡巴肼钠、嘧啶硫代苯甲酸酯、嘧啶肟和咪唑啉酮咪草烟具有抗性。这些结果表明G.所有这些突变导致了对5种ALS抑制除草剂的广泛交叉抗性。
Catchweed bedstraw (Galium aparine L.) is a problematic dicot weed that occurs in major winter wheat (Triticum aestivum L.) fields in China. Tribenuron-methyl has been widely used to control broadleaf weeds since 1988 in China. However, overuse has led to the resistance evolution of G. aparine to tribenuron-methyl. In this study, 20 G. aparine populations collected from Shandong and Henan provinces were used to determine tribenuron-methyl resistance and target-site resistance mechanisms. In dose-response experiments, 12 G. aparine populations showed different resistance levels (2.92 to 842.41-fold) to tribenuron-methyl compared with the susceptible population. Five different acetolactate synthase (ALS) mutations (Pro-197-Leu, Pro-197-Ser, Pro-197-His, Asp-376-Glu, and Trp-574-Leu) were detected in different resistant populations. Individuals heterozygous for Pro-197-Ser and Trp-574-Leu mutations were also observed in a resistant population (HN6). In addition, pHB4 (Pro-197-Ser), pHB7 (Pro-197-His), pHB8 (Pro-197-Leu), pHB5 (Asp-376-Glu), and pHB3 (Trp-574-Leu) subpopulations individually homozygous for specific ALS mutations were generated to evaluate the cross-resistance to ALS-inhibiting herbicides. The pHB4, pHB7, pHB8, pHB5, and pHB3 subpopulations all were resistant to sulfonylurea, pyrazosulfuron-ethyl, triazolopyrimidine, flumetsulam, sulfonylamino-carbonyl-triazolinone, flucarbazone-sodium, pyrimidinyl thiobenzoate, pyribenzoxim, and the imidazolinone imazethapyr. These results indicated the diversity of the resistance-conferring ALS mutations in G. aparine, and all these mutations resulted in broad cross-resistance to five kinds of ALS-inhibiting herbicides.