Improved method of differentiation, selection and amplification of human melanocytes from the hair follicle cell pool
Improved method of differentiation, selection and amplification of human melanocytes from the hair follicle cell pool
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DOI：
10.1111/exd.12038
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发表时间：
2012-12-01
影响因子：
3.6
通讯作者：
Simon, Jan C.
中科院分区：
文献类型：
--
作者：
Savkovic, Vuk;Dieckmann, Christina;Simon, Jan C.
发根拥有一个复杂的细胞池，具有巨大的发育潜力。毛囊外根鞘（ORS）的多能细胞可分化为多能细胞，包括皮肤细胞。外根鞘是已知的最丰富的非侵入性获得的成体干细胞来源。为了培养用于移植治疗脱色障碍的黑色素细胞，我们通过对Dieckmann的ORS外植体方法进行了几项方法学上的改进，建立了一种离体/体外培养方法。结果，我们在一半的时间内获得了更高纯度的分化黑色素细胞（4周内至少有106个95%纯度的细胞）。这种可靠的培养程序始于60根毛发的脱毛，并产生大量可用于移植应用的ORS黑素细胞。该方法不仅利用了毛根细胞池的发育潜力，有利于向黑素细胞分化，而且有利于微创到无创性的再生医学策略的发展。
Hair root harbours a complex cell pool with an immense developmental potential. Several lineages, including skin, can be differentiated from the multipotent to pluripotent cells of outer root sheath (ORS) of hair follicle. Outer root sheath presents the most opulent non-invasively gained adult stem cell source known. For the purposes of cultivating melanocytes designated for graft-based treatments of depigmentation disorders, we have established an ex vivo/in vitro cultivation method by introducing several methodological improvements to the ORS explant method of Dieckmann. As a result, we gained a higher, purer yield of differentiated melanocytes in half the time (at least 106 of 95% pure cells in 4 weeks). This reliable cultivation procedure begins with the epilation of 60 hairs and yields high numbers of ORS melanocytes that could be used for grafting applications. The procedure not only utilises the developmental potential of hair root cell pool and favors differentiation into melanocytes, but also contributes to the general trend of minimal-to-non-invasive strategies for regenerative medicine.