Correlations of neuronal and microvascular densities in murine cortex revealed by direct counting and colocalization of nuclei and vessels.
Correlations of neuronal and microvascular densities in murine cortex revealed by direct counting and colocalization of nuclei and vessels.
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DOI：
10.1523/jneurosci.3287-09.2009
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发表时间：
2009-11-18
期刊：
影响因子：
--
通讯作者：
Kleinfeld D
中科院分区：
文献类型：
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作者：
Tsai PS;Kaufhold JP;Blinder P;Friedman B;Drew PJ;Karten HJ;Lyden PD;Kleinfeld D
众所周知，在新皮层中，神经元的密度包括不同层之间的神经元密度以及不同区域之间的变化。 ，成像和分析工具，简单地绘制所有神经元和非神经元核的位置以及基于不同皮质区域的总库存测量值厚的新皮层的厚板中的所有血管的中心线和直径（〜107个细胞）在大脑中，这些方法揭示了：（1）在三个维度上，神经元的平均距离与最接近的微血管的平均距离为14μm。与神经元密度的层状变化相匹配。对于白质，在神经元和微血管密度之间显示出显着的相关性。皮质地幔。
It is well known that the density of neurons varies within the adult brain. In neocortex, this includes variations in neuronal density between different lamina as well as between different regions. Yet the concomitant variation of the microvessels is largely uncharted. Here we present automated histological, imaging, and analysis tools to simultaneously map the locations of all neuronal and non-neuronal nuclei and the centerlines and diameters of all blood vessels within thick slabs of neocortex from mice. Based on total inventory measurements of different cortical regions (~ 107 cells vectorized across brains), these methods revealed: (1) In three dimensions, the mean distance of the center of neuronal somata to the closest microvessel was 14 μm. (2) Volume samples within lamina of a given region show that the density of microvessels does not match the strong laminar variation in neuronal density. This holds for both agranular and granular cortex. (3) Volume samples in successive radii from the midline to the ventral-lateral edge, where each volume summed the number of cells and microvessels from the pia to the white matter, show a significant correlation between neuronal and microvessel densities. These data show that while neuronal and vascular densities do not track each other on the 100 μm scale of cortical lamina, they do track each other on the 1 – 10 mm scale of the cortical mantle. The absence of a disproportionate density of blood vessels in granular lamina is argued to be consistent with the initial locus of functional brain imaging signals.