Identification and characterization of a second novel human Erythrovirus variant, A6
Identification and characterization of a second novel human Erythrovirus variant, A6
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DOI：
10.1006/viro.2002.1585
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发表时间：
2002-09-30
期刊：
影响因子：
3.7
通讯作者：
Brown, KE
中科院分区：
文献类型：
--
作者：
Nguyen, QT;Wong, S;Brown, KE
细小病毒B19（Parvovirus B19，B19）是目前唯一被认可的红细胞病毒属成员，是已知的唯一对人类具有致病性的细小病毒。为了寻找额外的细小病毒变体，我们使用了新的NS 1/7.5EC PCR检测，其引物是从B19/V9序列的保守区域设计的，并且包含Mfel限制性内切酶位点，该位点可以区分B19和V9样序列。对225份血清和骨髓样本以及62份血浆池进行筛选，从一名贫血的HIV阳性患者中鉴定出一种新的非典型细小病毒序列A6，AS与B19的相似性为88%，与V9的相似性为92%，而报道的B19分离株之间的对应性>98%。开发了用于As衣壳转录物的RT-PCR，并用于测试UT 7/Epo/S1细胞的A6感染性。尽管病毒滴度高，但未检测到A6病毒转录物。因此，尽管B19变异的患病率可能很低，但真正的临床意义仍然未知。如果不设计针对A6/V9/B19共同序列的特异性引物，目前的PCR分析不太可能检测到新的变体。(C)2002 Elsevier Science（美国）。
Parvovirus B19 (B19), currently the only accepted member of the Erythrovirus genus, is the only parvovirus known to be pathogenic in humans, Recently a viral sequence, tentatively termed V9 which showed 11% variability from the published B19 sequences, was described from a patient with aplastic crisis. To search for additional parvovirus variants, we used the new NS1/7.5EC PCR assay whose primers were designed from a conserved region of the B19/V9 sequence and encompasses an Mfel restriction enzyme site that would allow differentiation between B19- and V9-like sequences. Screening of 225 serum and bone marrow samples and 62 plasma pools identified one new atypical parvovirus sequence, A6, from an anemic HIV-positive patient, AS exhibited 88% similarity to B19 and 92% to V9, compared to >98% correspondence between reported B19 isolates Based on the genome similarity to B19, an RT-PCR for As capsid transcripts was developed and used to test for A6 infectivity of UT7/Epo/S1 cells. Despite high viral titers, A6 viral transcripts were not detected. Thus, although the prevalence of B19 variants probably is low, the true clinical significance remains unknown. Current PCR analyses are unlikely to detect novel variants without the design of specific primers to the A6/V9/B19 common sequences. (C) 2002 Elsevier Science (USA).