Utilization of alkyne bioconjugations to modulate protein function
Utilization of alkyne bioconjugations to modulate protein function
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DOI：
10.1016/j.bmcl.2016.11.041
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发表时间：
2017-01-01
影响因子：
2.7
通讯作者：
Young, Douglas D.
中科院分区：
文献类型：
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作者：
Maza, Johnathan C.;Howard, Christina A.;Young, Douglas D.
引入或修饰蛋白质功能的能力广泛应用于多个科学学科。独特的非天然氨基酸的引入代表了引入新功能的极好机制;然而，这种方法受到翻译机制识别和引入化学部分的能力的限制。为了克服这种潜在的限制，我们的目标是利用现有的非天然氨基酸的功能来进行生物正交反应，以引入所需的蛋白质修饰，改变其功能。具体而言，通过引入含有非天然氨基酸的末端炔，我们证明了通过与其他末端炔的B-H偶联的化学可编程蛋白质修饰，改变了蛋白质的功能。在概念验证实验中，这种方法已被用来修改绿色荧光蛋白的荧光光谱。(C)2016爱思唯尔有限公司版权所有
The ability to introduce or modify protein function has widespread application to multiple scientific disciplines. The introduction of unique unnatural amino acids represents an excellent mechanism to incorporate new functionality; however, this approach is limited by ability of the translational machinery to recognize and incorporate the chemical moiety. To overcome this potential limitation, we aimed to exploit the functionality of existing unnatural amino acids to perform bioorthogonal reactions to introduce the desired protein modification, altering its function. Specifically, via the introduction of a terminal alkyne containing unnatural amino acid, we demonstrated chemically programmable protein modification through the Glaser-Hay coupling to other terminal alkynes, altering the function of a protein. In a proof-of-concept experiment, this approach has been utilized to modify the fluorescence spectrum of green fluorescent protein. (C) 2016 Elsevier Ltd. All rights reserved.