Isolation of an arbacia sperm fertilization antigen.
Isolation of an arbacia sperm fertilization antigen.
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阿尔巴西亚精子受精抗原的分离。
DOI：
10.1002/jez.1402210113
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Metz,CB
中科院分区：
文献类型：
--
作者：
Eckberg,WR;Metz,CB
抗精子抗体片段（IFab）通过抑制顶体反应和精子-卵子的附着来阻止海胆受精。我们在这里描述了一个68,000道尔顿的MW糖蛋白，它可以中和IFab的受精抑制作用。这种糖蛋白在精子膜的SDS -聚丙烯酰胺凝胶中是一种次要成分，但在精子膜的二碘多水杨酸锂提取物中含量很高。抗原的最终纯化是通过洗脱制备的SDS -聚丙烯酰胺凝胶来完成的。这种糖蛋白显然是海胆精子受体，它与卵冻膜相互作用，并作为顶体反应的“触发器”。
Antisperm antibody fragments (IFab) block sea urchin fertilization by inhibiting the acrosome reaction and consequently sperm‐egg attachment. We describe here a 68,000 dalton MW glycoprotein which neutralizes the fertilization‐inhibiting action of IFab. This glycoprotein is a minor component in SDS‐polyacrylamide gels of sperm membranes, but is greatly enriched in lithium diio‐dosalicylate extracts of the membranes. Final purification of the antigen was accomplished by elution from preparative SDS‐polyacrylamide gels. This glycoprotein is evidently the sea urchin sperm receptor which interacts with the egg jelly coat and acts as a “trigger” for the acrosome reaction.