Establishment and characterization of a fin cell line from Indian walking catfish, Clarias batrachus (L.).
Establishment and characterization of a fin cell line from Indian walking catfish, Clarias batrachus (L.).
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DOI：
10.1111/j.1365-2761.2011.01247.x
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发表时间：
2011-05
影响因子：
2.5
通讯作者：
V. S. Babu;K. Nambi;V. Chandra;V. P. Ishaq Ahmed;R. Bhonde;A. S. Sahul Hameed
中科院分区：
文献类型：
--
作者：
V. S. Babu;K. Nambi;V. Chandra;V. P. Ishaq Ahmed;R. Bhonde;A. S. Sahul Hameed
建立了一个新的细胞系，印度鲶鱼鳍，来自印度步行鲶鱼，胡子鲶鳍组织，并进行了表征。该细胞系在补充有15%胎牛血清（FBS）的Leibovitz L-15培养基中生长良好，自2007年启动以来已传代培养110多次。细胞能够在28至37 °C的温度范围内生长，最佳生长温度为28 °C。该细胞系主要由成纤维细胞样细胞组成。在28 °C下，随着FBS浓度从2%增加到20%，鳍细胞的生长速率增加，其中在15%或20%的浓度下生长最佳，而在5%的浓度下生长较差。发现细胞对鱼诺达病毒和IPNV-ab敏感，并通过细胞病变效应和逆转录酶-聚合酶链反应证实感染。线粒体12 SrRNA的PCR扩增，使用特异性引物的C。Batrachus证实了该细胞系的鲶鱼起源。通过免疫细胞化学、pEGFP-N1转染效率和荧光激活细胞分选的细胞周期分析进一步表征细胞系。
A new cell line, Indian Catfish Fin, derived from the fin tissue of Indian walking catfish, Clarias batrachus, was established and characterized. The cell line grew well in Leibovitz's L-15 medium supplemented with 15% foetal bovine serum (FBS) and has been subcultured more than 110 times since its initiation in 2007. The cells were able to grow at a range of temperature from 28 to 37 °C with optimal growth at 28 °C. The cell line predominantly consists of fibroblast-like cells. The growth rate of fin cells increased as the FBS concentration increased from 2% to 20% at 28 °C with optimum growth at a concentration of 15% or 20% and poor growth at a concentration of 5%. The cells were found to be susceptible to fish nodavirus and IPNV-ab and infection was confirmed by cytopathic effect and reverse transcriptase-polymerase chain reaction. PCR amplification of mitochondrial 12S rRNA using primers specific to C. batrachus confirmed the catfish origin of the cell line. The cell line was characterized further by immunocytochemistry, transfection efficiency with pEGFP-N1 and cell cycle analysis by fluorescent-activated cell sorting.