Molecular Mechanism of Transcriptional Cascade Initiated by the EvgS/EvgA System in Escherichia coli K-12
Molecular Mechanism of Transcriptional Cascade Initiated by the EvgS/EvgA System in Escherichia coli K-12
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DOI：
10.1271/bbb.80795
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发表时间：
2009-04-01
影响因子：
1.6
通讯作者：
Utsumi, Ryutaro
中科院分区：
文献类型：
--
作者：
Itou, Junji;Eguchi, Yoko;Utsumi, Ryutaro
使用EvgS活性突变体（evgSI）与基因缺失相结合，我们阐明了大肠杆菌中由EvgS/EvgA双组分系统启动的耐酸性和多药耐药基因转录级联的分子机制，随后依次诱导转录调节因子YdeO和GadE。已知EvgA（EvgS/EvgA系统的反应调节剂）的过表达诱导许多耐酸性和多药耐药基因的表达，其中涉及EvgA-YdeO-GadE回路，但传感器EvgS在该回路中的作用仍未解决。我们的研究结果表明，转录级联启动的EvgS/EvgA系统实际上功能的酸和药物抗性在大肠杆菌。杆菌
Using an EvgS-active mutant (evgSI) in combination with gene deletions, we clarified the molecular mechanism of the transcriptional cascade of acid resistance and multidrug resistance genes initiated by the EvgS/EvgA two-component system in Escherichia coli, followed by sequential induction of the transcriptional regulators, YdeO and GadE. Overexpression of EvgA, the response regulator of the EvgS/EvgA system, is known to induce the expression of a number of acid resistance and multidrug resistance genes, in which the EvgA-YdeO-GadE circuit is involved, but the role of the sensor EvgS in this circuit has remained unsolved. Our results suggest that the transcriptional cascade initiated by the EvgS/EvgA system in fact functions for acid and drug resistance in E. coli.