Hepatoprotective effect of Quercetin supplementation against Acrylamide-induced DNA damage in wistar rats
Hepatoprotective effect of Quercetin supplementation against Acrylamide-induced DNA damage in wistar rats
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DOI：
10.1186/s12906-016-1322-7
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发表时间：
2016-08-30
影响因子：
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通讯作者：
Abudawood, Manal
中科院分区：
文献类型：
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作者：
Ansar, Sabah;Siddiqi, Nikhat Jamal;Abudawood, Manal
背景：槲皮素 (QR) 是一种多酚类黄酮化合物，在某些食物中大量存在，可防止氧化应激。本研究旨在确定槲皮素是否可能对丙烯酰胺（ACR）引起的大鼠毒性发挥保肝和抗氧化活性。方法：四组Wistar大鼠，每组六只大鼠：（i）对照组； (ii) ACR治疗组(50毫克/千克体重)； (iii)QR组：用QR(10mg/kg体重)治疗大鼠； (iv) 腹膜内给予 QR (10 mg/kg bw)。持续 5 天，然后在第 5 天进行 ACR（50 毫克/千克体重）（单剂量）。结果：ACR 导致 8-OH 鸟苷水平升高和谷胱甘肽 S-转移酶 (GST) 活性降低。除一些组织病理学改变指标外，QR 的施用可显着保护肝脏组织免受丙烯酰胺的肝毒性作用，从而改善标记酶 (p < 0.05) 和 DNA 损伤 (p < 0.01)，这通过彗星试验证明，此外还有一些组织病理学改变。 结论：得出的结论是，QR 可以保护肝脏免受 ACR 诱导的 DNA 损伤，因此可能能够改善 ACR 诱导的大鼠肝脏变化。
Background: Quercetin (QR), is a polyphenolic flavonoid compound which is found in large amounts in certain foods, and protects against oxidative stress. The current study was conducted to determine whether Quercetin can possibly exert hepatoprotective and antioxidant activity against acrylamide (ACR) induced toxicity in rats.Methods: Four groups of Wistar rats consisting of six rats each: (i) control group; (ii) ACR treated group (50 mg/kg bw); (iii) QR group: rats were treated with QR (10 mg/kg bw); (iv) QR (10 mg/kg bw) was given i.p. for 5 days followed by ACR (50 mg/kg bw) on 5th day (single dose).Results: ACR caused an elevation in 8-OH guanosine level and a reduction in Glutahione S-transferase (GST) activity. Administration of QR significantly protected liver tissue against hepatotoxic effect of acrylamide from amelioration of the marker enzyme (p < 0.05) and DNA damage (p < 0.01) as evident by comet assay and, besides some indices of histopathological alterations.Conclusion: It is concluded that QR could protect the liver against DNA damage induced by ACR probably is thus capable of ameliorating ACR-induced changes in the rat livers.