Improved nanomechanical and in-vitro biocompatibility of graphene oxide-carbon nanotube hydroxyapatite hybrid composites by synergistic effect
Improved nanomechanical and in-vitro biocompatibility of graphene oxide-carbon nanotube hydroxyapatite hybrid composites by synergistic effect
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DOI：
10.1016/j.jmbbm.2021.104376
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发表时间：
2021-02-19
影响因子：
3.9
通讯作者：
Kumar，Navin
中科院分区：
文献类型：
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作者：
Jyoti，Jeevan;Kiran，Abhimanyu;Kumar，Navin
羟基磷灰石（Hydroxyapatite， HAP）具有与天然磷灰石相似的物理化学性质，是一种具有新骨生长、硬组织修复、生物活性、成骨细胞粘附和增殖等功能的生物材料。HAP固有的脆性和较差的力学性能限制了其潜在的临床应用。利用碳纳米填料（碳纳米管（CNTs）、氧化石墨烯（GO）、氧化石墨烯-碳纳米管（GCNTs）杂化）的独特性能，制备碳纳米填料基HAP复合材料。研究了碳纳米填料增强HAP复合材料的纳米力学性能和体外生物相容性。碳纳米填料增强HAP复合材料的纳米力学性能和生物相容性得到改善。GCNTs-HAP复合材料的纳米压痕硬度和弹性模量明显高于其他碳纳米填料增强复合材料和原始HAP粉末。采用mtt法对制备的碳纳米填料增强HAP复合材料的细胞毒性进行了检测。与其他碳纳米填料和HAP相比，制备的含有2% GCNTs纳米填料的GCNTs-HAP复合材料具有更高的细胞活力、更好的相容性和更强的细胞增殖诱导能力。这些发现将为氧化石墨烯及其复合材料在骨修复、再生、增强和植入等方面的应用提供新的前景。
Hydroxyapatite (HAP) is an attractive bio-material for new bone growth process, hard tissue repair, bioactivity, osteoblast adhesion and proliferation due to its physicochemical resembles natural apatite. The intrinsic brittleness and poor mechanical properties of HAP restrict it for potential clinical applications. This problem is undertaken by exploiting the unique properties of carbon nanofillers (carbon nanotube (CNTs), graphene oxide (GO), graphene oxide-carbon nanotube (GCNTs) hybrid) which are used as reinforcement for preparing the carbon nanofillers based HAP composites. The nanomechanical andin-vitrobiocompatibility of carbon nanofiller reinforced HAP composites have been studied. Carbon nanofiller reinforced HAP composites led to an improvement in nanomechanical and biocompatibility properties. The nanoindentation hardness and elastic modulus of GCNTs-HAP composites are significantly higher than other carbon nanofiller reinforced composites and pristine HAP powder. Thein-vitrocytotoxicity of the prepared carbon nanofillers reinforced HAP composites is examined using MTT-assay on the MDCK cell line. The prepared GCNTs-HAP composites containing 2% of GCNTs nanofiller show higher cell viability, improved compatibility, and superior one cell proliferation induction than the other carbon nanofillers and HAP. These findings will provide the new prospects for utilizing the GO and its hybrid in HAP composites in bone repair, regeneration, augmentation and implantation.