Oligonucleotide-selenide conjugate: Synthesis and its inducible sequence-specific alkylation of DNA
Oligonucleotide-selenide conjugate: Synthesis and its inducible sequence-specific alkylation of DNA
复制标题
寡核苷酸-硒化物缀合物：DNA 的合成及其诱导序列特异性烷基化
DOI：
10.1016/j.bmc.2009.11.026
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发表时间：
2010-06-01
影响因子：
3.5
通讯作者：
Constant, Jean-Francois
中科院分区：
文献类型：
--
作者：
Du, Yuhao;Weng, Xiaocheng;Constant, Jean-Francois
设计并合成了寡核苷酸-硒偶联物，研究了其序列特异性交联能力。硒化物衍生物可通过高碘酸盐氧化形成o-QM中间体与互补链发生共价链间交联。获得了高达50%的交联反应产率。羟基自由基足迹实验表明，醌附属物特异性地烷基化胞嘧啶碱基，延伸缀合物和靶链之间形成的双链体。(C)2009爱思唯尔有限公司保留所有权利。
Oligonucleotide-selenium conjugate was designed and synthesized and its sequence-specific cross-linking ability was investigated. The selenide derivatives can generate covalent interstrand cross-linking with its complementary strand through the formation of o-QM intermediate induced by periodate oxidation. A cross-linking reaction yield of up to 50% was obtained. Hydroxyl radical footprinting experiment revealed that the quinone appendage specifically alkylated the cytosine base extending the duplex formed between the conjugate and the target strand. (C) 2009 Elsevier Ltd. All rights reserved.