Differential morphological changes in sympathetic nerve fibers elicited by lead, cadmium, and mercury.
Differential morphological changes in sympathetic nerve fibers elicited by lead, cadmium, and mercury.
复制标题
铅、镉和汞引起的交感神经纤维的不同形态变化。
DOI：
10.1016/0013-9351(81)90184-5
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发表时间：
1981
影响因子：
8.3
通讯作者：
Seiger,A
中科院分区：
文献类型：
--
作者：
Björklund,H;Hoffer,B;Olson,L;Seiger,A
用单胺荧光组织化学方法研究了眼内注射醋酸铅、氯化镉或氯化汞对交感肾上腺素能虹膜地面神经丛的影响。与注射醋酸钠的对照虹膜相比，前房注射5μL 1.4 mM铅溶液可引起明显的过度神经支配。这种超神经支配在注射后2周持续存在。将剂量增加到42 mM并不增加神经支配的程度。氯化镉溶液(1.4 mM和7 mM)对肾上腺素能神经纤维密度无影响。注射氯化汞可引起肾上腺素能神经明显变性，且呈剂量依赖关系。注射3.5毫米汞氯化汞溶液两周后，神经几乎完全再生。我们的结果表明，眼内注射神经毒性重金属，并随后用Falck-Hillarp荧光显微镜进行分析，是评估肾上腺素能神经毒性的一种快速而敏感的方法。
Effects of intraocular injection of lead acetate, cadmium chloride, or mercury chloride on the sympathetic adrenergic ground plexus of the iris were studied using monoamine fluorescence histochemistry. Injection of 5 μl of a 1.4 mmlead solution into the anterior eye chamber caused a significant hyperinnervation as compared to sodium acetate-injected control irides. This hyperinnervation persisted 2 weeks after injection. Increasing the dose to 42 mmdid not increase the extent of the hyperinnervation. Cadmium chloride solutions (1.4 and 7 mm) did not affect the adrenergic nerve fiber density as compared to sodium chloride-injected controls. Injection of mercury chloride caused a marked and dose-dependent degeneration of adrenergic nerves. Two weeks after injection of a 3.5 mmmercury chloride solution, the nerves had regenerated almost completely. Our results demonstrate that intraocular injections of neurotoxic heavy metals, and subsequent analysis with Falck-Hillarp fluorescence microscopy, is a rapid and sensitive method to evaluate adrenergic neurotoxicity.