Regional distribution of the prostaglandin E2 receptor EP1 in the rat brain -: Accumulation in Purkinje cells of the cerebellum
Regional distribution of the prostaglandin E2 receptor EP1 in the rat brain -: Accumulation in Purkinje cells of the cerebellum
复制标题
DOI：
10.1385/jmn:27:3:303
复制
发表时间：
2005-01-01
影响因子：
3.1
通讯作者：
Fiebich, BL
中科院分区：
文献类型：
--
作者：
Candelario-Jalil, E;Slawik, H;Fiebich, BL
前列腺素E-2(PGE(2))是一种主要的前列腺素类化合物，由环氧合酶(COX)响应各种生理和病理刺激而产生。 PGE2 通过激活四种特定的 E 型前列腺素受体（EP1、EP2、EP3 和 EP4）发挥作用。在本研究中，我们采用逆转录聚合酶链反应、蛋白质印迹和免疫组化方法分析了成年大鼠大脑不同区域（海马、下丘脑、纹状体、前额叶大脑皮层、顶叶皮层、脑干和小脑）中PGE2受体EP1（mRNA和蛋白）的表达。从区域来看，顶叶皮层和小脑的 EP1 nRNA 水平最高。在蛋白质水平上，我们发现小脑中 EP1 的表达非常强，正如蛋白质印迹实验所揭示的那样。此外，本研究首次提供了证据表明 EP1 受体在小脑中高度表达，正如免疫组织化学分析中所观察到的，浦肯野细胞在其核周和树突上显示出非常高的免疫标记。本研究的结果表明，EP1 前列腺素受体在特定神经元群体中表达，这可能决定对 PGE(2) 的区域特异性反应。
Prostaglandin E-2(PGE(2)), is a major prostanoid produced by the activity of cyclooxygenases (COX) in response to various physiological and pathological stimuli. PGE2 exerts its effects by activating four specific E-type prostanoid receptors (EP1, EP2, EP3, and EP4). In the present study, we analyzed the expression of the PGE2 receptor EP1 (mRNA and protein) in different regions of the adult rat brain (hippocampus, hypothalamus, striatum, prefrontal cerebral cortex, parietal cortex, brain stem, and cerebellum) using reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemical methods. On a regional basis, levels of EP1 nRNA were the highest in parietal cortex and cerebellum. At the protein level, we found very strong expression of EP1 in cerebellum, as revealed by Western blotting experiments. Furthermore, the present study provides for the first time evidence that the EP1 receptor is highly expressed in the cerebellum, where the Purkinje cells displayed very high immunolabeling of their perikaryon and dendrites, as observed in the immunohistochemical analysis. Results from the present study indicate that the EP1 prostanoid receptor is expressed in specific neuronal populations, which possibly determine the region-specific response to PGE(2).