Complete sequence of the 45-kb mouse ribosomal DNA repeat: analysis of the intergenic spacer
Complete sequence of the 45-kb mouse ribosomal DNA repeat: analysis of the intergenic spacer
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DOI：
10.1016/s0888-7543(03)00199-x
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发表时间：
2003-12-01
期刊：
影响因子：
4.4
通讯作者：
Karagyozov, L
中科院分区：
文献类型：
--
作者：
Grozdanov, P;Georgiev, O;Karagyozov, L
使用来自单个细菌人工染色体克隆的DNA对小鼠核糖体DNA基因间间隔区从45S前体RNA的3’末端到间隔区启动子进行测序（登录号AF 441733）。这使得完整的小鼠核糖体DNA重复单元（45，309bp长，TPA登录号BK 000964）的组装成为可能。基因间隔区（IGS）的分析表明，高密度的简单重复序列和转座元件。IGS包含两个串联重复的长序列块。这些模块中的一些序列也存在于IGS的其他部分中。观察到小鼠IGS突变率沿着存在差异。序列基序的IGS转录增强，转录终止，复制起点，和核仁组织的意义进行了讨论。(C)2003年爱思唯尔公司All rights reserved.
DNA from a single bacterial artificial chromosome clone was used to sequence the mouse ribosomal DNA intergenic spacer from the 3' end of the 45S pre-RNA to the spacer promoter (Accession No. AF441733). This made possible the assembly of a complete mouse ribosomal DNA repeat unit (45,309 bp long, TPA Accession No. BK000964). Analysis of the intergenic spacer (IGS) showed a high density of simple sequence repeats and transposable elements. The IGS contains two long sequence blocks, which are repeated tandemly. Some of the sequences in these blocks are also present in other parts of the IGS. A difference in the mutation rate along the mouse IGS was observed. The significance of sequence motifs in the IGS for transcription enhancement, transcription termination, origin of replication, and nucleolar organization is discussed. (C) 2003 Elsevier Inc. All rights reserved.