Differential expression of the CB2 cannabinoid receptor by rodent macrophages and macrophage-like cells in relation to cell activation
Differential expression of the CB2 cannabinoid receptor by rodent macrophages and macrophage-like cells in relation to cell activation
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DOI：
10.1016/s1567-5769(01)00147-3
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发表时间：
2002-01-01
影响因子：
5.6
通讯作者：
Cabral, GA
中科院分区：
文献类型：
--
作者：
Carlisle, SJ;Marciano-Cabral, F;Cabral, GA
采用一种多步骤激活的体外模型来评估大麻素受体的表达，在该模型中，巨噬细胞系的细胞依次被驱动通过炎症、致敏和完全激活状态。小鼠和大鼠的腹腔巨噬细胞、小鼠RAW264.7和P388D(1)巨噬细胞样细胞以及新生大鼠大脑皮质小胶质细胞的大麻素受体2型（CB2）表达与细胞激活相关且存在差异。CB2在常驻腹腔巨噬细胞中无法检测到，在巯基乙酸盐诱导的炎症和干扰素γ（IFN -γ）致敏的腹腔巨噬细胞中高水平存在，而在细菌脂多糖（LPS）激活的腹腔巨噬细胞中检测到的水平显著降低。在小鼠巨噬细胞样细胞和新生大鼠大脑皮质小胶质细胞中也观察到了CB2类似的差异表达模式。无论细胞激活状态如何，在腹腔巨噬细胞或小鼠巨噬细胞样细胞中均未检测到大麻素受体1型（CB1），但在新生大鼠小胶质细胞中低水平存在。这些结果表明，巨噬细胞系细胞中CB2的水平随着细胞激活发生重大的调节性变化。此外，由于炎症和致敏的巨噬细胞表达最高水平的CB2，处于这些相应激活状态的巨噬细胞的功能活性可能对大麻素的作用最为敏感。（C）2002爱思唯尔科学出版社。保留所有权利。
An in vitro model of multi-step activation, in which cells of macrophage lineage are driven sequentially through inflammatory, primed, and fully activated states, was employed to assess for cannabinoid receptor expression. Murine and rat peritoneal macrophages, murine RAW264.7 and P388D(1) macrophage-like cells, and neonatal rat brain cortex microglia expressed the cannabinoid receptor type 2 (CB2) differentially in relation to cell activation. The CB2 was undetectable in resident peritoneal macrophages, present at high levels in thioglycolate-elicited inflammatory and interferon gamma (IFN gamma)-primed peritoneal macrophages, and detected at significantly diminished levels in bacterial lipopolysaccharide (LPS)-activated peritoneal macrophages. A comparable pattern of differential expression of the CB2 was noted for murine macrophage-like cells and neonatal rat brain cortex microglia. The cannabinoid receptor type 1 (CB1) was not detected in peritoneal macrophages or murine macrophage-like cells regardless of cell activation state but was present in neonatal rat microglia at low levels. These results indicate that levels of the CB2 in cells of macrophage lineage undergo major modulatory changes in relation to cell activation. Furthermore, since inflammatory and primed macrophages express the highest levels of CB2, the functional activities of macrophages when in these respective states of activation may be the most sensitive to the action of cannabinoids. (C) 2002 Elsevier Science B.V. All rights reserved.