Serodiagnosis of louse-borne relapsing fever with glycerophosphodiester phosphodiesterase (GlpQ) from Borrelia recurrentis
Serodiagnosis of louse-borne relapsing fever with glycerophosphodiester phosphodiesterase (GlpQ) from Borrelia recurrentis
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DOI：
10.1128/jcm.38.10.3561-3571.2000
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发表时间：
2000-10-01
影响因子：
9.4
通讯作者：
Schwan, TG
中科院分区：
文献类型：
--
作者：
Porcella, SF;Raffel, SJ;Schwan, TG
人虱传回归热在非洲中部和东部发生零星暴发，其特征是显著的发病率和死亡率。分离的病原体，复发性疏螺旋体，从血液中的四个病人在最近的流行病在苏丹南部。对这些分离株的glpQ基因（编码甘油磷酸二酯磷酸二酯酶）进行测序，并与从其他回归热螺旋体获得的glpQ序列进行比较。以前我们表明，GlpQ的赫氏疏螺旋体是一种免疫原性蛋白与实用程序作为血清学试验抗原，用于区分蜱传回归热前莱姆病。本研究从B中克隆并表达了glpQ基因。并在血清学试验中使用重组GlpQ。急性期和恢复期血清：采用间接免疫荧光试验（IFA）和酶联免疫吸附试验（ELISA）对42例虱传回归热患者的样本进行检测，其中使用B全细胞。复发性和免疫印迹的螺旋体和大肠杆菌产生重组GlpQ的全细胞裂解物。IFA法测定的急性期和恢复期血清样品的几何平均滴度分别为1：83和1：575。免疫印迹分析确定了高水平的反应性和血清转化GlpQ，和测定比全细胞IFA和ELISA使用纯化的，重组组氨酸标记的GlpQ更敏感。血清抗体GlpQ和其他抗原持续27西尔斯在一个病人。我们的结论是，抗GlpQ抗体的评估将允许虱传回归热的血清学确认和确定疾病的患病率。
Human louse-borne relapsing fever occurs in sporadic outbreaks ih central and eastern Africa that are characterized by significant morbidity and mortality. Isolates of the causative agent, Borrelia recurrentis, were obtained from the blood of four patients during a recent epidemic of the disease in southern Sudan. The glpQ gene, encoding glycerophosphodiester phosphodiesterase, from these isolates was sequenced and compared with the glpQ sequences obtained from other relapsing-fever spirochetes. Previously we showed that GlpQ of Borrelia hermsii is an immunogenic protein with utility as a serological test antigen for discriminating tick borne relapsing fever front Lyme disease. In the present work, we Cloned and expressed the glpQ gene from B. recurrentis and used recombinant GlpQ in serological tests. Acute- and convalescent-phase serum: samples obtained from 42 patients with louse-borne relapsing fever were tested with an indirect immunofluorescence assay (IFA) and an enzyme-linked immunosorbent assay (ELISA) that used whole cells of B. recurrentis and with immunoblotting to whole-cell lysates of the spirochete and Escherichia: coli producing recombinant GlpQ. The geometric mean titers of the acute- and Convalescent-phase serum samples measured by IFA were 1:83 and 1:575, respectively. The immunoblot analysis identified a high level of reactivity and seroconversion to GlpQ, and the assay was more sensitive than the whole-cell IFA and ELISA using purified, recombinant histidine-tagged GlpQ. Serum antibodies to GlpQ and other antigens persisted for 27 Sears in one patient. We conclude that assessment of anti-GlpQ antibodies will allow serological confirmation of louse-borne relapsing fever and determination of disease prevalence.