Anti-β-glucan antibodies in healthy human subjects
Anti-β-glucan antibodies in healthy human subjects
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DOI：
10.1016/j.vaccine.2008.11.030
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发表时间：
2009-01-22
期刊：
影响因子：
5.5
通讯作者：
Torosantucci, A.
中科院分区：
文献类型：
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作者：
Chiani, P.;Bromuro, C.;Torosantucci, A.
我们小组先前的数据证明了由海带多糖（β-（13）-葡聚糖）组成的疫苗的抗真菌功效，与白喉类毒素结合，在小鼠中产生保护性抗海带多糖抗体。在这篇论文中，我们在一群健康的人类受试者中寻找天然抗海带多糖抗体的存在、同种型和亚类组成，并与抗β-（1，6）-葡聚糖（pustulan）和支链β-（1，3/1，6）-葡聚糖（池1）以及来自白色念珠菌的甘露聚糖的抗体进行比较。几乎所有受试者都显示出可检测水平的抗β-葡聚糖抗体，IgG主要占IgM的比例，伊加很少（如果有的话），没有IgE。然而，抗β-葡聚糖抗体的滴度总体上比相应同种型的抗甘露聚糖抗体的滴度低约1 log。特别是，抗海带多糖IgG的水平最低，其几何平均滴度（95%置信区间，CI）为1838（1245-2714），而pustulan和Pool I真菌葡聚糖分别为8157（6067- 10，931）和3940（2911-5332）。IgG亚类组成分析表明，IgG 2是主要的亚类，抗任何抗原，抗海带多糖IgG 2的浓度最低，其几何平均浓度为0.13（0.07-0.24）μ g/ml，而抗脓疱多糖和抗池1葡聚糖和甘露聚糖IgG 2水平为0.33（0.2-0.5）、1.35（0.9-2.0）和36.1（25.2-51.3）μ g/ml。这些数据表明，与其他抗β-葡聚糖抗体和大多数抗甘露聚糖抗体相比，抗海带多糖抗体在人体中的水平较低，并表明人体中的保护性抗真菌疫苗接种应试图使抗真菌抗体的平衡偏向抗海带多糖抗体。(C)2008爱思唯尔有限公司版权所有。
Previous data by Our group demonstrated the antifungal efficacy of a vaccine consisting of laminarin (beta-(13)-glucan), Conjugated with diphtheria toxoid, which generated protective anti-laminarin antibodies in mice. In this paper, we sought for the presence, isotype and subclass composition of natural anti-laminarin antibodies in an unselected population of human healthy Subjects, in a comparison with antibodies directed against beta-(1,6)-glucan (pustulan) and branched beta-(1,3/1,6)-glucan (Pool 1) and mannan from Candida albicans. Almost all subjects showed detectable levels of anti-p-glucan antibodies, with IgG largely prevailing on IgM, little, if any, IgA and no IgE. However, the titer of anti-beta-glucan antibodies was overall about I log lower than that of anti-mannan antibodies of the corresponding isotype. In particular, the level of anti-laminarin IgG was the lowest one, its geometrical mean titer (95% confidence interval, Cl) being 1838 (1245-2714) as compared to 8157 (6067-10,931) and 3940 (2911-5332) for pustulan and Pool I fungal glucan, respectively. Analysis of IgG Subclass composition showed that IgG2 was the prevalent subclass, against any antigen, and again the concentration of anti-laminarin IgG2 was the lowest one, its geometrical mean concentration being 0.13 (0.07-0.24) mu g/ml as compared to anti-pustulan and anti-Pool 1 glucan and mannan IgG2 levels, which were 0.33 (0.2-0.5), 1.35 (0.9-2.0), and 36.1 (25.2-51.3) mu g/ml, respectively. These data show that anti-laminarin antibodies are present at low levels in humans as compared to other anti-beta-glucan and, mostly, anti-mannan antibodies, and suggest that a protective antifungal vaccination in humans Should attempt to tip the balance of antifungal antibodies in favour of the anti-laminarin ones. (C) 2008 Elsevier Ltd. All rights reserved.