Quantitative analysis of the cellular inflammatory response against biofilm bacteria in chronic wounds
Quantitative analysis of the cellular inflammatory response against biofilm bacteria in chronic wounds
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DOI：
10.1111/j.1524-475x.2011.00681.x
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发表时间：
2011-05-01
影响因子：
2.9
通讯作者：
Tolker-Nielsen, Tim
中科院分区：
文献类型：
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作者：
Fazli, Mustafa;Bjarnsholt, Thomas;Tolker-Nielsen, Tim
慢性伤口是一个世界性的重要问题。这些创面的特点是持续的炎症阶段，伴随着中性粒细胞的过度聚集和细胞活性的升高，这表明必须有持续的刺激来吸引和招募中性粒细胞到伤口。其中一个刺激因素可能是慢性伤口中细菌生物膜的存在。本研究以小腿慢性静脉性溃疡活检标本为研究对象，应用基于核酸的荧光原位杂交技术(PNA-FISH)和激光共聚焦扫描显微镜进行细菌检测。伤口中的细菌通常聚集在大团块中。为了测量慢性创面对细菌的细胞炎症反应，通过对含有铜绿假单胞菌或金黄色葡萄球菌的创面组织切片进行中性粒细胞计数，评估了感染部位积累的中性粒细胞的数量。与含金黄色葡萄球菌的创面相比，含铜绿假单胞菌创面的中性粒细胞积累量显著增加。这些结果与铜绿假单胞菌生物膜在慢性创面中的存在可能是导致持续性炎症反应和创面愈合不良的主要因素之一的假说有关。
Chronic wounds are an important problem worldwide. These wounds are characterized by a persistent inflammatory stage associated with excessive accumulation and elevated cell activity of neutrophils, suggesting that there must be a persistent stimulus that attracts and recruits neutrophils to the wound. One such stimulus might be the presence of bacterial biofilms in chronic wounds. In the present study, biopsy specimens from chronic venous leg ulcers were investigated for the detection of bacteria using peptide nucleic acid-based fluorescence in situ hybridization (PNA-FISH) and confocal laser scanning microscopy. The bacteria in the wounds were often situated in large aggregates. To obtain a measure of the cellular inflammatory response against the bacteria in the chronic wounds, the amount of neutrophils accumulated at the site of infection was evaluated through differential neutrophil counting on the tissue sections from wounds containing either Pseudomonas aeruginosa or Staphylococcus aureus. The P. aeruginosa-containing wounds had significantly higher numbers of neutrophils accumulated compared with the S. aureus-containing wounds. These results are discussed in relation to the hypothesis that the presence of P. aeruginosa biofilms in chronic wounds may be one of the main factors leading to a persistent inflammatory response and impaired wound healing.