Myosin binding protein C phosphorylation in normal, hypertrophic and failing human heart muscle
Myosin binding protein C phosphorylation in normal, hypertrophic and failing human heart muscle
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DOI：
10.1016/j.yjmcc.2008.05.020
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发表时间：
2008-08-01
影响因子：
5
通讯作者：
Marston, Steven B.
中科院分区：
文献类型：
--
作者：
Jacques, Adam M.;Copeland, O'Neal;Marston, Steven B.
研究了肥厚型心肌病患者室间隔肌切除术后肌球蛋白结合蛋白C（MyBP-C）的磷酸化。将这些样品与供体心肌（作为良好表征的对照组织）和终末期衰竭心肌进行比较。使用Hartzell和Glass的磷酸盐-EDTA提取的修改从肌原纤维部分纯化MyBP-C。通过SDS-PAGE分离MyBP-C，并使用Pro-Q Diamond染色磷蛋白，然后使用考马斯蓝染色总蛋白。相对磷酸化水平由MyBP-C条带的Pro-Q Diamond与考马斯蓝染色的比率确定，如通过光密度测定法测量的。我们比较了9例心肌切除术样本和9例衰竭心脏样本与9例供体样本。病理肌肉中MyBP-C磷酸化水平低于供体（供体肌肉切除40 +/- 2%，P
Phosphorylation of myosin binding protein C (MyBP-C) was investigated in intraventricular septum samples taken from patients with hypertrophic cardiomyopathy undergoing surgical septal myectomy. These samples were compared with donor heart muscle, as a well-characterised control tissue, and with end-stage failing heart muscle. MyBP-C was partly purified from myofibrils using a modification of the phosphate-EDTA extraction of Hartzell and Glass. MyBP-C was separated by SDS-PAGE and stained for phosphoproteins using Pro-Q Diamond followed by total protein staining using Coomassie Blue. Relative phosphorylation level was determined from the ratio of Pro-Q Diamond to Coomassie Blue staining of MyBP-C bands as measured by densitometry. We compared 9 myectomy samples and 9 failing heart samples with 9 donor samples. MyBP-C phosphorylation in pathological muscle was lower than in donor (myectomy 40 +/- 2% of donor, P