Regeneration of dental pulp following pulpectomy by fractionated stem/progenitor cells from bone marrow and adipose tissue
Regeneration of dental pulp following pulpectomy by fractionated stem/progenitor cells from bone marrow and adipose tissue
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DOI：
10.1016/j.biomaterials.2011.11.056
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发表时间：
2012-03-01
期刊：
影响因子：
14
通讯作者：
Nakashima, Misako
中科院分区：
文献类型：
--
作者：
Ishizaka, Ryo;Iohara, Koichiro;Nakashima, Misako
牙髓干/祖细胞可诱导牙髓完全再生。然而，由于随着年龄的增长，牙髓组织的可用性有限，因此需要检查其他来源的侧群（SP）细胞的分数。在本研究中，将同一个体的骨髓和脂肪组织作为替代来源进行评估。牙髓CD 31（-）SP细胞比骨髓和脂肪CD 31（-）SP细胞具有更高的迁移活性和更高的血管生成/神经营养因子表达。脂肪组织CD 31（-）SP细胞移植产生与牙髓衍生细胞相同量的再生组织。然而，骨髓CD 31（-）SP细胞移植产生显着较少的再生组织在狗的牙髓切除根管。在第28天，脂肪CD 31（-）SP细胞移植的基质形成率远高于牙髓CD 31（-）SP细胞移植。微阵列分析表明，从所有三种细胞来源的再生组织中牙髓的mRNA表达特征的定性和定量模式相似。移植细胞中许多血管生成/神经营养因子的表达表现出营养作用。我们的研究结果表明，骨髓和脂肪CD 31（-）SP细胞可能是合适的替代细胞来源的牙髓再生。(C)2011爱思唯尔有限公司保留所有权利。
Pulp stem/progenitor cells can induce complete pulp regeneration. However, due to the limited availability of pulp tissue with age, there is a need to examine other sources for fractions of side population (SP) cells. In the present investigation bone marrow and adipose tissues of the same individual were evaluated as alternate sources. Pulp CD31(-) SP cells have higher migration activity and higher expression of angiogenic/neurotrophic factors than bone marrow and adipose CD31(-) SP cells. Adipose tissue CD31(-) SP cell transplantation yielded the same amount of regenerated tissue as pulp derived cells. However, bone marrow CD31(-) SP cell transplantation yielded significantly less regenerated tissue in pulpectomized root canals in dogs. The rate of matrix formation was much higher in adipose CD31(-) SP cell transplantation compared to pulp CD31(-) SP cell transplantation on day 28. Microarray analysis demonstrated similar qualitative and quantitative patterns of mRNA expression characteristic of pulp in the regenerated tissues from all three cell sources. Expression of many angiogenic/neurotrophic factors in the transplanted cells demonstrated trophic effects. Our results demonstrate that bone marrow and adipose CD31(-) SP cells might be suitable alternative cell sources for pulp regeneration. (C) 2011 Elsevier Ltd. All rights reserved.