Wnt-1 Protein as a Prognostic Biomarker for Hepatitis B-Related and Hepatitis C-Related Hepatocellular Carcinoma after Surgery
Wnt-1 Protein as a Prognostic Biomarker for Hepatitis B-Related and Hepatitis C-Related Hepatocellular Carcinoma after Surgery
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DOI：
10.1158/1055-9965.epi-09-0039
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发表时间：
2009-05-01
影响因子：
3.8
通讯作者：
Wang, Andrew H-J.
中科院分区：
文献类型：
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作者：
Lee, Hao-Hsien;Uen, Yih-Huei;Wang, Andrew H-J.
背景资料：Wnt-1蛋白在与B型肝炎病毒（HBV）和丙型肝炎病毒（HCV）相关的肝细胞癌（HCC）组织和细胞系中表达上调。它是已知的信号癌症进展中发挥着重要作用，而其在HCC的预后作用仍然unexplored.Methods：作为一个预后的生物标志物，本研究分析了Wnt-1蛋白表达在63个组织学验证的肝癌患者接受根治性切除术。在每个配对的肿瘤和非肿瘤标本中，Wnt-1水平通过Western印迹法半定量测量，并以肿瘤/非肿瘤比率表示。这些数据进一步与定量实时PCR以及β-连环蛋白和E-钙粘蛋白的表达免疫组织化学。结果：结果显示，26例（第1组）和37例（第11组）HCC患者Wnt-1表达率>= 1.5，
Background: Up-regulation of Wnt-1 protein has been reported in hepatitis B virus (HBV)-related and hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC) tissues and cell lines. It is known to play a fundamental role in signaling cancer progression, whereas its prognostic role in HCC remains unexplored.Methods: As a prognostic biomarker, this study analyzed Wnt-1 protein expression in 63 histology-verified HCC patients receiving curative resection. In each paired tumor and nontumor specimen, Wnt-1 levels were semi quantitatively measured by Western blotting and expressed by tumor/nontumor ratio. The data were further correlated with quantitative real-time PCR as well as with beta-catenin and E-cadherin expression by immunohistochemistry. Cumulative tumor recurrence-free survival curves were constructed using the Kaplan-Meier method and compared by the log-rank test.Results: The results showed that 26 (group 1) and 37 (group 11) HCC patients had an expression ratio of Wnt-1 >= 1.5 and