A smart nanosensor for the detection of human immunodeficiency virus and associated cardiovascular and arthritis diseases using functionalized graphene-based transistors
A smart nanosensor for the detection of human immunodeficiency virus and associated cardiovascular and arthritis diseases using functionalized graphene-based transistors
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DOI：
10.1016/j.bios.2018.11.041
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发表时间：
2019-02-01
影响因子：
12.6
通讯作者：
Gandhi, Sonu
中科院分区：
文献类型：
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作者：
Islam, Saurav;Shukla, Shruti;Gandhi, Sonu
人类免疫缺陷病毒（HIV）伊萨个全球性的公共卫生问题，通常与心血管疾病（CVD）和类风湿性关节炎（RA）有关。开发了一种智能纳米传感器，用于使用石墨烯基场效应晶体管（FET）检测HIV及其相关疾病（CVD和RA）。在这项研究中，胺官能化石墨烯（afG）与抗体[抗p24用于HIV，抗心肌肌钙蛋白1（抗cTn 1）用于CVD，抗环瓜氨酸肽（抗CCP）用于RA]结合，以检测各种生物标志物。通过碳二亚胺活化将抗体共价缀合至afG。生物缀合物（石墨烯-抗体）通过各种生物物理技术表征，例如UV-Vis、拉曼光谱、扫描电子显微镜（SEM）和原子力显微镜（AFM）。相对于由于抗原与其特异性抗体的相互作用而引起的电极表面电阻的变化来评价传感器的电化学性能。开发的传感器是高度敏感的，并显示出线性响应p24，cTn 1，和，CCP从1 fg/mL至1 μ g/mL。在标准优化条件下，p24的检测限（LOD）为100 fg/mL，cTn 1和CCP的检测限为10 fg/mL。基于石墨烯的智能纳米器件表现出优异的性能;因此，它可用于现场检测真实的样品中的HIV、CVD和RA生物标志物。
Human immunodeficiency virus (HIV), which isa worldwide public health issue, is commonly associated with cardiovascular disorders (CVDs) and rheumatoid arthritis (RA). A smart nanosensor was developed for the detection of HIV and its related diseases (CVDs and RA) using graphene-based field-effect transistors (FETs). In this study, amine-functionalized graphene (afG) was conjugated with antibodies [anti-p24 for HIV, anti-cardiac troponin 1 (anti-cTn1) for CVDs, and anti-cyclic citrullinated peptide (anti-CCP) for RA] to detect various biomarkers. The antibodies were covalently conjugated to afG via carbodiimide activation. The bioconjugate (graphene-antibody) was characterized by various biophysical techniques such as UV-Vis, Raman spectroscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). The electrochemical performance of the sensor was evaluated with respect to changes in the resistance of the electrode surface due to the interaction of the antigen with its specific antibody. The developed sensor was highly sensitive and showed a linear response to p24, cTn1, and, CCP from 1 fg/mL to 1 mu g/mL. The limit of detection (LOD) was 100 fg/mL for p24 and 10 fg/mL for cTn1 and CCP under standard optimized conditions. The graphene-based smart nanodevice demonstrated excellent performance; thus, it could be used for the on-site detection of HIV, CVD, and RA biomarkers in real samples.