Relationship between Trypanosoma cruzi and human chagasic megaesophagus:: Blood and tissue parasitism
Relationship between Trypanosoma cruzi and human chagasic megaesophagus:: Blood and tissue parasitism
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DOI：
10.4269/ajtmh.2001.65.435
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发表时间：
2001-11-01
影响因子：
3.3
通讯作者：
Chiari, E
中科院分区：
文献类型：
--
作者：
Lages-Silva, E;Crema, E;Chiari, E
研究了52例慢性恰加斯病消化道型患者组织和血液中克氏锥虫的持续存在。这些患者患有巨食管返流并接受了矫正手术。在分析中使用了寄生虫学（异种诊断、血培养或两者）、组织病理学（苏木精和伊红以及过氧化物酶-抗过氧化物酶染色）和分子（聚合酶链反应[PCR]，然后进行狭缝印迹杂交）检测。T.在98%（51/52）的患者中可检测到Cruzi、其基因组片段或其抗原。食管组织中随机检出率为76.9%，PCR检出率为90.4%，血液中寄生虫学检出率为73.1%。50%（52个中的26个）的组织样本有炎症，其中80.8%与寄生虫有关。克氏锥虫也被鉴定为与炎症改变无关。在IV级巨食管患者的食管组织中观察到较高的组织寄生和强烈的炎症过程。这些数据表明，在消化系统形式的恰加斯病，特别是在巨食管的情况下，T。克氏杆菌经常在血液和组织中发现，并可能导致相关的致病机制。
The persistence of Trypanosoma cruzi in tissue and blood of 52 patients in the digestive form of chronic Chagas disease was studied. These patients had chagasic megaesophagus and underwent corrective surgery. Parasitologic (xenodiagnosis, hemoculture, or both), histopathologic (hematoxylin and eosin, and peroxidase-anti-peroxidase staining), and molecular (polymerase chain reaction [PCR] followed by slot-blot hybridization) tests were used in the analysis. The presence of T. cruzi, its genomic fragments, or its antigens could be detected in 98% (51 of 52) of the patients. The parasite was randomly identified in 76.9% of esophageal tissues and in 90.4% by PCR and in 73.1% by parasitologic methods from the blood. Fifty percent (26 of 52) of tissue samples had inflammation, 80.8% of which was associated with the parasite. Ttypanosoma cruzi was also identified unassociated with inflammatory alterations. Higher tissue parasitism and intense inflammatory processes were observed in esophageal tissue from patients with Grade IV megaesophagus. These data demonstrate that in the digestive form of Chagas' disease, particularly in cases of megaesophagus, T. cruzi is frequently found, both in blood and tissues and may contribute to the pathogenic mechanisms involved.