Functional, Histopathological and Immunohistichemical Assessments of Cyclosporine A on Sciatic Nerve Regeneration Using Allografts: A Rat Sciatic Nerve Model.
Functional, Histopathological and Immunohistichemical Assessments of Cyclosporine A on Sciatic Nerve Regeneration Using Allografts: A Rat Sciatic Nerve Model.
复制标题
使用同种异体移植物进行环孢素 A 对坐骨神经再生的功能、组织病理学和免疫组织化学评估：大鼠坐骨神经模型。
DOI：
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发表时间：
2017
影响因子：
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通讯作者：
R. Mohammadi
中科院分区：
文献类型：
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作者：
A. Amniattalab;R. Mohammadi
目标
目的：研究环孢菌素A对大鼠坐骨神经移植后功能、组织病理学和免疫组织化学的影响。
方法
雄性Wistar大鼠30只，随机分为正常对照组(NC)、同种异体移植组(Allo)、环孢素A(CsA)治疗组(Allo/CsA)，每组10只。NC组，左侧坐骨神经经臀肌切开显露后，动态肌缝合。在Allo组，左侧坐骨神经经臀肌切开显露，横断胫腓分叉部近端，切除10 mm。Allo/CsA组采用同样的方法，同时给予环孢素A。Allo组大鼠的切取神经作为Allo/CsA组的同种异体神经移植，反之亦然。对照组和Allo组给予300μL无菌橄榄油，每日1次，共1周；Allo/CsA组给予300μL CsA(1 mg/kg/d)，每日1次，共1周。
结果
在行为、功能、生物力学和腓肠肌质量方面，Allo/CsA组轴突再生早于Allo组(p=0.001)。组织形态计量学和免疫组织化学研究也显示Allo/CsA组轴突再生早于Allo组(p=0.034)。
结论
CsA可促进同种异体坐骨神经移植后的功能恢复。对急诊神经切断术后患者的手术治疗有一定的临床指导意义。
OBJECTIVES
To study the functional, histopathological and immunohistochemical effect of cyclosporine A on sciatic nerve regeneration using allografts in a rat sciatic nerve model.
METHODS
Thirty male white Wistar rats were divided into three experimental groups (n = 10), randomly: Normal control group (NC), allograft group (ALLO), CsA treated group (ALLO/ CsA). In NC group left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In the ALLO group the left sciatic nerve was exposed through a gluteal muscle incision and transected proximal to the tibio-peroneal bifurcation where a 10 mm segment was excised. The same procedure was performed in the ALLO/ CsA group and the animals were treated with interaperitoneal administration of cyclosporine A. The harvested nerves of the rats of ALLO group were served as allograft for ALLO/ CsA group and vice versa. The NC and ALLO groups received 300 μL sterile olive oil interaperitoneally once a day for one week and the ALLO/ CsA group received 300 μL CsA (1mg/kg/day) interaperitoneally once a day for one week.
RESULTS
Behavioral, functional, biomechanical and gastrocnemius muscle mass showed earlier regeneration of axons in ALLO/ CsA than in ALLO group (p=0.001). Histomorphometic and immunohistochemical studies also showed earlier regeneration of axons in ALLO/ CsA than in ALLO group (p=0.034).
CONCLUSION
Administration of CsA could accelerate functional recovery after nerve allografting in sciatic nerve. It may have clinical implications for the surgical management of patients after nerve transection in emergency conditions.