ABSENCE OF RESTRICTED DIFFUSION IN ADIPOSE-TISSUE CAPILLARIES
ABSENCE OF RESTRICTED DIFFUSION IN ADIPOSE-TISSUE CAPILLARIES
复制标题
DOI：
10.1111/j.1748-1716.1977.tb05967.x
复制
发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
PAASKE, WP
中科院分区：
文献类型：
--
作者：
PAASKE, WP
采用单次注射外挂片法测定了~(57)Co-氰基钴胺(57Co-B12)在兔脂肪组织中的毛细血管通透性。毛细管扩散容量CDC(渗透性表面积乘积，PS)为1.1ml/100g.cntdot。在毛细管提取0.21，血浆流量6.7ml/100g·cntdot的条件下，最小检测时间为0.21分钟。最小。将结果与采用类似方法和制备的先前研究的51Cr-EDTA数据进行比较。当CDC(51Cr-EDTA)/CDC(57Co-B12)为1.81时，D(51Cr-EDTA)/D(57Co-B12)在水中的自由扩散系数之比为37。C，为1.79。与51Cr-EDTA相比，57Co-B12在皮肤组织中没有发生限制扩散，即51Cr-EDTA和57Co-B12在脂肪组织毛细血管膜上的扩散速度与它们在水中的自由扩散系数成正比。Pappenheimer等效孔半径估计为30°和Karnovsky内皮间缝隙宽度为40°都不能很好地解释实验数据。融合囊泡的跨内皮通道系统可能是本研究结果的一个结构上的等价物。连续型毛细血管无论位于哪个组织，都表现出相似的渗透特性。
Capillary permeability in rabbit adipose tissue for 57Co-cyanocobalamin (57Co-B12) was determined by the single injection, external registration method. The capillary diffusion capacity, CDC, (the permeability-surface area product, PS) was 1.1 ml/100 g .cntdot. min at a capillary extraction of 0.21 and a plasma flow of 6.7 ml/100 g .cntdot. min. Results were compared to 51Cr-EDTA data from a previous study with similar method and preparation. As CDC(51Cr-EDTA)/CDC(57Co-B12) was 1.81 and as D(51Cr-EDTA)/D(57Co-B12), the ratio between the free diffusion coefficients in water at 37.degree. C, was 1.79. Restricted diffusion does not occur in cutaneous tissue for 57Co-B12 as compared to 51Cr-EDTA, i.e., 51Cr-EDTA and 57Co-B12 diffuse across the capillary membrane of adipose tissue at rates proportional to their respective free diffusion coefficients in water. The Pappenheimer equivalent pore radius estimate of 30 .ANG. and the Karnovsky interendothelial 40 .ANG. slit width are both defective in explaining the experimental data. The transendothelial channel system of fused vesicles is a possible structural equivalent for the present findings. Capillaries of continuous type exhibit similar permeation characteristics regardless of the tissue in which they are located.