Single-neuron diversity generated by Protocadherin-β cluster in mouse central and peripheral nervous systems.
Single-neuron diversity generated by Protocadherin-β cluster in mouse central and peripheral nervous systems.
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DOI：
10.3389/fnmol.2012.00090
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发表时间：
2012
影响因子：
4.8
通讯作者：
Yagi T
中科院分区：
文献类型：
--
作者：
Hirano K;Kaneko R;Izawa T;Kawaguchi M;Kitsukawa T;Yagi T
复杂神经回路的产生依赖于具有不同个体特征的神经元的正确连接。为了了解神经系统的复杂性，必须阐明确定单个神经元的同一性和多样性的分子机制。哺乳动物的聚集型原钙粘附素(Pcdh)由大约50个Pcdh基因(Pcdh-α、Pcdh-β和Pcdh-γ)组成，编码钙粘素家族细胞表面黏附蛋白。单个神经元表达Pcdh-α和Pcdh-γ的随机组合，而Pcdh-β基因-22个单外显子基因在小鼠中的表达模式尚不完全清楚。在这里，我们发现Pcdh-β基因在小鼠脑中以3‘-多聚腺苷酸化的形式表达。使用PAN-Pcdh-β探针与保守的Pcdh-β序列进行原位杂交显示，大脑中有广泛的标记，在嗅球、海马体和小脑中有明显的信号。与单个Pcdh-β基因的特异性探针的原位杂交显示，它们在P10至P150的浦肯野细胞中的表达是分散的。通过对浦肯野细胞进行的新发展的单细胞3‘-RACE分析证实了分散的表达模式，这清楚地表明Pcdh-β基因在单个浦肯野细胞中以单等位和组合的方式表达。Pcdh-β基因在海马和大脑皮质的锥体神经元、三叉神经节和背根神经节的神经元、GABA能中间神经元和胆碱能神经元中也有分散的表达模式。我们的结果扩展了之前对Pcdh表达产生的单个神经元水平上的多样性的观察，并表明Pcdh-β簇基因有助于指定单个神经元的身份和多样性。
The generation of complex neural circuits depends on the correct wiring of neurons with diverse individual characteristics. To understand the complexity of the nervous system, the molecular mechanisms for specifying the identity and diversity of individual neurons must be elucidated. The clustered protocadherins (Pcdh) in mammals consist of approximately 50 Pcdh genes (Pcdh-α, Pcdh-β, and Pcdh-γ) that encode cadherin-family cell surface adhesion proteins. Individual neurons express a random combination of Pcdh-α and Pcdh-γ, whereas the expression patterns for the Pcdh-β genes, 22 one-exon genes in mouse, are not fully understood. Here we show that the Pcdh-β genes are expressed in a 3′-polyadenylated form in mouse brain. In situ hybridization using a pan-Pcdh-β probe against a conserved Pcdh-β sequence showed widespread labeling in the brain, with prominent signals in the olfactory bulb, hippocampus, and cerebellum. In situ hybridization with specific probes for individual Pcdh-β genes showed their expression to be scattered in Purkinje cells from P10 to P150. The scattered expression patterns were confirmed by performing a newly developed single-cell 3′-RACE analysis of Purkinje cells, which clearly demonstrated that the Pcdh-β genes are expressed monoallelically and combinatorially in individual Purkinje cells. Scattered expression patterns of individual Pcdh-β genes were also observed in pyramidal neurons in the hippocampus and cerebral cortex, neurons in the trigeminal and dorsal root ganglion, GABAergic interneurons, and cholinergic neurons. Our results extend previous observations of diversity at the single-neuron level generated by Pcdh expression and suggest that the Pcdh-β cluster genes contribute to specifying the identity and diversity of individual neurons.
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将CTCF鉴定为聚集的原钙粘着蛋白基因的主要调节剂。
影响因子：
14.9
作者：
Golan-Mashiach M;Grunspan M;Emmanuel R;Gibbs-Bar L;Dikstein R;Shapiro E
通讯作者：
Shapiro E
影响因子：
4.8
作者：
Kawaguchi, Masahumi;Toyama, Tomoko;Yagi, Takeshi
通讯作者：
Yagi, Takeshi
影响因子：
3.4
作者：
Fukuda, Emi;Hamada, Shun;Yagi, Takeshi
通讯作者：
Yagi, Takeshi
影响因子：
7
作者：
Han, Meng-Hsuan;Lin, Chengyi;Wang, Xiaozhong
通讯作者：
Wang, Xiaozhong
影响因子：
3.4
作者：
Junghans, Dirk;Heidenreich, Matthias;Kemler, Rolf
通讯作者：
Kemler, Rolf