Development of ultrafast laser-based x-ray in-vivo phase-contrast micro-CT beamline for biomedical applications at Advanced Laser Light Source (ALLS).
Development of ultrafast laser-based x-ray in-vivo phase-contrast micro-CT beamline for biomedical applications at Advanced Laser Light Source (ALLS).
复制标题
开发基于超快激光的 X 射线体内相衬微型 CT 光束线，用于先进激光光源 (ALLS) 的生物医学应用。
DOI：
10.1117/12.795542
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Pogany,Andrew
中科院分区：
文献类型：
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作者：
Kincaid,Russell;Krol,Andrzej;Fourmaux,Sylvain;Kieffer,Jean-Claude;Serbanescu,Cristina;Servol,Marina;Vogelsang,Levon;Wilkins,Steve;Stevenson,Andrew;Nesterets,Yakov;Lipson,Edward;Ye,Hongwei;Pogany,Andrew
我们正在开发和探索的成像性能，在体内，同轴全息，X射线相衬，微CT系统与超快激光为基础的X射线（ULX）源。通过测试和改进我们的系统，并通过进行计算机模拟，我们计划提高系统性能的对比度分辨率和多能量成像的水平超出了可以使用传统的微焦点X射线管。在菲涅尔方案中采集单能量（Mo Kα和Kβ线）的初始CT投影集，并对体模和安乐死小鼠进行重建。我们还进行了计算机模拟相衬显微CT扫描低对比度，软组织，肿瘤成像。我们确定，为了使用ULX执行相位对比完整的显微CT扫描，必须满足以下条件：（i）X射线源在扫描期间需要稳定;（ii）对于大于30 cm的源到物体距离，激光焦斑尺寸需要小于10 μm;（iii）目标上的激光强度需要在5 × 1017至5 × 1019 W/cm 2的范围内;（iv）烧蚀保护系统需要允许不间断的扫描;（v）在整个扫描过程中，聚焦在目标上的激光必须保持准确;（vi）在整个扫描过程中，目标的新表面必须暴露于连续的激光照射;（vii）有效探测器元件尺寸必须小于12 μm。基于在该研究项目中获得的结果，我们预计，新的10 Hz，200 TW的激光与50 W的平均功率，正在委托在ALLS将使我们能够在体内的X射线相衬显微CT的实际实施。
We are developing and exploring the imaging performance of, an in vivo, in-line holography, x-ray phase-contrast, micro-CT system with an ultrafast laser-based x-ray (ULX) source. By testing and refining our system, and by performing computer simulations, we plan to improve system performance in terms of contrast resolution and multi-energy imaging to a level beyond what can be obtained using a conventional microfocal x-ray tube. Initial CT projection sets at single energy (Mo Kα and Kβ lines) were acquired in the Fresnel regime and reconstructed for phantoms and a euthanized mouse. We also performed computer simulations of phase-contrast micro-CT scans for low-contrast, soft-tissue, tumor imaging. We determined that, in order to perform a phase-contrast, complete micro-CT scan using ULX, the following conditions must be met: (i) the x-ray source needs to be stable during the scan; (ii) the laser focal spot size needs to be less than 10 μm for source-to-object distance greater than 30 cm; (iii) the laser light intensity on the target needs to be in the range of 5 × 1017 to 5 × 1019 W/cm2; (iv) the ablation protection system needs to allow uninterrupted scans; (v) the laser light focusing on the target needs to remain accurate during the entire scan; (vi) a fresh surface of the target must be exposed to consecutive laser shots during the entire scan; (vii) the effective detector element size must be less than 12 μm. Based on the results obtained in this research project, we anticipate that the new 10 Hz, 200 TW laser with 50 W average power that is being commissioned at ALLS will allow us practical implementation of in vivo x-ray phase-contrast micro-CT.