SURGICAL AND PHARMACOLOGICAL DISSOCIATION OF CARDIOVASCULAR AND EMETIC RESPONSES TO INTRAGASTRIC CUSO4
SURGICAL AND PHARMACOLOGICAL DISSOCIATION OF CARDIOVASCULAR AND EMETIC RESPONSES TO INTRAGASTRIC CUSO4
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DOI：
10.1152/ajpregu.1992.263.2.r284
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发表时间：
1992-08-01
影响因子：
--
通讯作者：
KING, GL
中科院分区：
文献类型：
--
作者：
MAKALE, MT;KING, GL
在控制（完整的和剖腹）条件下，动脉血压的ESTA麻醉雪貂上升显着响应于胃内硫酸铜（CuSO 4; 10毫升的5毫克/毫升）。CuSO 4在75-90%的时间内是呕吐性的，并且心血管反应总是立即在呕吐反应之前并伴随呕吐反应。预先给予六甲双铵（0.35mg/kg）和甲基溴化阿托品（1 mg/kg）可明显减轻或消除心血管反应。在六甲双铵和阿托品组合中加入α-2-受体拮抗剂2，3-二氯-α-甲基苄胺盐酸盐（DCMB，10 mg/kg）和β-受体拮抗剂普萘洛尔（3 mg/kg）可防止其降低心血管反应。单独给药，这些药物不会改变心血管反应，育亨宾（0.30 mg/kg）、RS（+/-）-扎考必利（0.30 mg/kg）或格拉司琼（0.50 mg/kg）也不会改变心血管反应。仅RS（+/-）-扎考必利显著降低呕吐发生率。单独使用甲基溴化阿托品，与六甲双铵，或与六甲双铵，普萘洛尔和DCMB显着延迟第一次呕吐发作。相反，双侧腹部迷走神经切断术显著减少了呕吐动物的数量，而不影响心血管反应。这些结果表明，介导这两个事件的神经通路是不相同的。在另一系列的实验中，一个显着更大比例的动物呕吐，在胃内CuSO 4比十二指肠内CuSO 4，这表明在雪貂的CuSO 4行动的主要网站是胃。
Under control (intact and laparotomized) conditions, arterial pressure of urethan-anesthetized ferrets rose significantly in response to intragastric copper sulfate (CuSO4; 10 ml of 5 mg/ml). CuSO4 was emetic 75-90% of the time, and a cardiovascular response always immediately preceded and accompanied emetic responses. The cardiovascular response was significantly reduced or abolished by hexamethonium (0.35 mg/kg) pretreatment combined with atropine methyl bromide (1 mg/kg). Addition of the alpha-2-receptor antagonist 2,3-dichloro-alpha-methylbenzylamine HCl (DCMB, 10 mg/kg) and the beta-receptor antagonist propranolol (3 mg/kg) to the hexamethonium and atropine combination prevented its reduction of the cardiovascular response. Given individually, these agents did not alter cardiovascular response, nor did yohimbine (0.30 mg/kg), RS(+/-)-zacopride (0.30 mg/kg), or granisetron (0.50 mg/kg). Only RS(+/-)-zacopride significantly reduced incidence of emesis. Atropine methyl bromide alone, with hexamethonium, or with hexamethonium, propranolol, and DCMB significantly delayed the first emetic episode. Conversely, bilateral abdominal vagotomy significantly reduced the number of vomiting animals without affecting cardiovascular response. These results suggest that the neural pathways mediating the two events are not identical. In another series of experiments, a significantly greater proportion of animals vomited in response to intragastric CuSO4 than to intraduodenal CuSO4, suggesting that the primary site of CuSO4 action in the ferret is the stomach.