REGULATION OF INTERLEUKIN-1 RECEPTOR EXPRESSION IN MOUSE-BRAIN AND PITUITARY BY LIPOPOLYSACCHARIDE AND GLUCOCORTICOIDS
REGULATION OF INTERLEUKIN-1 RECEPTOR EXPRESSION IN MOUSE-BRAIN AND PITUITARY BY LIPOPOLYSACCHARIDE AND GLUCOCORTICOIDS
复制标题
DOI：
10.1159/000126594
复制
发表时间：
1993-11-01
影响因子：
4.1
通讯作者：
HAOUR, F
中科院分区：
文献类型：
--
作者：
BAN, E;MARQUETTE, C;HAOUR, F
白细胞介素-1（IL-1）受体已在小鼠垂体和脑中得到表征。以前的研究表明，IL-1受体密度高，在海马齿状回和脂多糖（LPS）注射引起海马IL-1受体的数量减少80%，而垂体前叶的垂体受体不受影响。为了探讨糖皮质激素（GC）在脑和垂体IL-1受体表达调控中的作用，测定了应激、外源性GC给药和肾上腺切除术（ADX）对IL-1受体密度的影响。以重组人I-125-IL-1 α为示踪剂，采用原位定量放射自显影技术，在基础和LPS刺激条件下进行测定。固定应激或地塞米松（DEX）治疗（短期和长期）后，血清中GC浓度的增加并没有改变海马中基础条件下或外周LPS注射后IL-1受体的密度。相反，ADX显著降低LPS处理动物的IL-1受体密度。在垂体前叶，基础IL-1受体的密度显着增加，观察6小时后制动应力或7天后的DEX治疗，而短期DEX治疗无效。与在海马中观察到的相反，在ADX小鼠中没有观察到垂体受体密度的变化。这些结果表明，海马和垂体IL-1受体表达的差异调节GC。因此，这份报告构成了第一个演示的IL-1受体在垂体中的体内调节。
Interleukin-1 (IL-1) receptors have been characterized in mouse pituitary and brain. Previous studies have demonstrated that IL-1 receptor density is high in the dentate gyrus in the hippocampus and that lipopolysaccharide (LPS) injection caused an 80% decrease in the number of hippocampal IL-1 receptors, while pituitary receptors in the anterior pituitary were unaffected. In order to investigate on the role of glucocorticoids (GC) in the control of IL-1 receptor expression in the brain and pituitary, the effect of stress, exogenous GC administration or adrenalectomy (ADX) on IL-1 receptor density was determined. Assays were achieved under basal and LPS-stimulated conditions by in situ quantitative autoradiography technique using human recombinant I-125-IL-1alpha as a tracer. An increase of GC concentration in serum, following immobilization stress or dexamethasone (DEX) treatment (short and long term), did not modify, in the hippocampus, the density of IL-1 receptors under basal conditions or after peripheral LPS injection. On the contrary, ADX significantly decreased IL-1 receptor density in LPS-treated animals. In the anterior pituitary, a significant increase in the density of basal IL-1 receptors was observed 6 h following immobilization stress or after 7 days of DEX treatment while short-term DEX treatments are ineffective. In contrast to what was observed in the hippocampus, no changes in pituitary receptor densities were observed in ADX mice. These results indicate that hippocampal and pituitary IL-1 receptor expressions are differentially regulated by GC. Therefore, this report constitutes the first demonstration of an in vivo regulation of IL-1 receptors in the pituitary.