Cadherin Fragments from Anopheles gambiae Synergize Bacillus thuringiensis Cry4Ba's Toxicity against Aedes aegypti Larvae
Cadherin Fragments from Anopheles gambiae Synergize Bacillus thuringiensis Cry4Ba's Toxicity against Aedes aegypti Larvae
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DOI：
10.1128/aem.01870-09
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发表时间：
2009-11-15
影响因子：
4.4
通讯作者：
Adang, Michael J.
中科院分区：
文献类型：
--
作者：
Park, Youngjin;Hua, Gang;Adang, Michael J.
报道冈比亚按蚊幼虫钙粘蛋白AgCad1多肽是苏云金芽孢杆菌亚种的增效剂。以色列冷库蚊对按蚊的毒性(G.hua，R.Zhang，M.A.Abdullah，和M.J.Adang，生物化学47：5101-5110,2008)。我们报道了膜近端胞外区(MPED)的CR11(CR11-MPED)和来自AgCad1的较长的多肽CR9到CR11(CR9-11)是Cry4BA对埃及伊蚊幼虫毒性的增效剂，但基于Cry3(Y.Park，M.A.F.Abdullah，M.D.Taylor，K.Rahman和M.J.Adang，Appl.)的增效剂。环境。微生物。75：3086-3092,2009)不影响Cry4BA的毒性。CR9-11和CR11-MPED以高亲和力(分别为13 nM和23 nM)与Cry4BA结合，并抑制Cry4BA与埃及斑潜蝇幼虫刷状缘膜的结合。较长的CR9-11片段比CR11-MPED更能增强Cry4BA对埃及伊蚊的抗性。
A peptide from cadherin AgCad1 of Anopheles gambiae larvae was reported as a synergist of Bacillus thuringiensis subsp. israelensis Cry4Ba's toxicity to the Anopheles mosquito (G. Hua, R. Zhang, M. A. Abdullah, and M. J. Adang, Biochemistry 47: 5101-5110, 2008). We report that CR11 to the membrane proximal extracellular domain (MPED) (CR11-MPED) and a longer peptide, CR9 to CR11 (CR9-11), from AgCad1 act as synergists of Cry4Ba's toxicity to Aedes aegypti larvae, but a Diabrotica virgifera virgifera cadherin-based synergist of Cry3 (Y. Park, M. A. F. Abdullah, M. D. Taylor, K. Rahman, and M. J. Adang, Appl. Environ. Microbiol. 75: 3086-3092, 2009) did not affect Cry4Ba's toxicity. Peptides CR9-11 and CR11-MPED bound Cry4Ba with high affinity (13 nM and 23 nM, respectively) and inhibited Cry4Ba binding to the larval A. aegypti brush border membrane. The longer CR9-11 fragment was more potent than CR11-MPED in enhancing Cry4Ba against A. aegypti.