PLANAR DIFFERENCES IN NUCLEAR-AREA AND ORIENTATION IN THE SUBVENTRICULAR AND INTERMEDIATE ZONES OF THE RAT EMBRYONIC NEOCORTEX
PLANAR DIFFERENCES IN NUCLEAR-AREA AND ORIENTATION IN THE SUBVENTRICULAR AND INTERMEDIATE ZONES OF THE RAT EMBRYONIC NEOCORTEX
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DOI：
10.1002/cne.903070311
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发表时间：
1991-05-15
影响因子：
2.5
通讯作者：
HUMPHREYS, L
中科院分区：
文献类型：
--
作者：
BAYER, SA;ALTMAN, J;HUMPHREYS, L
在背内侧新皮质的冠状面与矢状面上定量研究了室下区和中间区的核面积和方向。 在胚胎日（E）13和E22之间研究了Nissl染色的甲基丙烯酸酯包埋的正常大鼠胚胎。 用计算机绘图程序确定核轮廓的面积及其长轴（定义为通过核轮廓中最远的两个点的直线）偏离水平（定义为平行于软脑膜）的程度。 由于细胞核是胚胎细胞中轮廓最清晰的结构，因此细胞核的面积和方向反映了细胞体的整体大小和方向。 核面积在冠状面比在矢状面大，特别是在E17和E20之间。 室管膜下和下中间区的细胞体取向在冠状面主要是水平的，在矢状面主要是垂直的。 在上中间区，细胞体的方向主要是垂直在两个平面，但更是在矢状面。 这些数据表明，室下区和下中间区的大多数细胞体具有水平取向的扁平椭圆形，其较大直径位于冠状面内，而其较小直径位于矢状面内。 由于扁平化，细胞体在矢状面中错误地表现为垂直取向。 水平切片的定性观察证实了定量计算机分析。 这些结果与[H-3]胸苷放射自显影有关细胞迁移和细胞在室管膜下和中间区的逗留的其他发现有关。
Nuclear area and orientation in the subventricular and intermediate zones was studied quantitatively in coronal vs. sagittal sections of the dorsomedial neocortex. Nissl-stained methacrylate-embedded normal rat embryos were studied between embryonic days (E) 13 and E22. The area of nuclear profiles and the degrees their long axes (defined as a straight line through the two most distant points in the nuclear profile) deviated from the horizontal (defined as parallel to the pial membrane) were determined with a computer-graphics program. Because the nucleus is the most clearly outlined structure in embryonic cells, the area and orientation of the nucleus was taken to reflect the overall size and orientation of the cell body. Nuclear area is larger in the coronal plane than it is in the sagittal plane, especially between E17 and E20. Cell body orientation in the subventricular and lower intermediate zones is predominantly horizontal in the coronal plane and predominantly vertical in the sagittal plane. In the upper intermediate zone, cell body orientation is predominantly vertical in both planes, but more so in the sagittal plane. These data indicate that the majority of cell bodies in the subventricular and lower intermediate zones have a horizontally oriented, flattened elliptical shape with their larger diameters lying within the coronal plane and their smaller diameters in the sagittal plane. Because of the flattening, the cell bodies falsely appear to be vertically oriented in the sagittal plane. Qualitative observations in horizontal sections confirmed the quantitative computer analysis. These results are related to other findings with [H-3]thymidine autoradiography concerning cell migration and the sojourn of cells in the subventricular and intermediate zones.