Heterologous expression of bacterial nitric oxide synthase gene: a potential biological method to control biofilm development in the environment.
Heterologous expression of bacterial nitric oxide synthase gene: a potential biological method to control biofilm development in the environment.
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细菌一氧化氮合酶基因的异源表达：控制环境中生物膜发育的潜在生物学方法。
DOI：
10.1139/w11-141
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发表时间：
2012
影响因子：
2.8
通讯作者：
Wenli Chen
中科院分区：
文献类型：
--
作者：
Pulin Liu;Qiaoyun Huang;Wenli Chen
利用广谱表达载体在恶臭假单胞菌中异源表达枯草芽孢杆菌一氧化氮合酶基因。一氧化氮特异性荧光探针的性能和一氧化氮终产物的定量评估都证明了一氧化氮的产生。内源性一氧化氮增加了恶臭假单胞菌的运动性，并降低了恶臭假单胞菌和其他多种细菌形成生物膜的能力。在实践方面，内源性一氧化氮在产生连续的、可控水平的一氧化氮方面提供了优势，这表明了一种使用一氧化氮控制生物膜的新方法。
The nitric oxide synthase gene from Bacillus subtilis was heterologously expressed in Pseudomonas putida using a broad-host expression vector. Both the performance of the nitric oxide-specific fluorescent probe and the quantitative assessment of the nitric oxide end products demonstrated the generation of nitric oxide. The endogenous nitric oxide increased the motility of P. putida and decreased the capacity of P. putida and other multispecies bacteria to develop biofilms. On the practical side, endogenous nitric oxide offers an advantage in generating continuous, controllable levels of nitric oxide, which suggests a new method to use nitric oxide in the control of biofilms.
影响因子：
3.6
作者：
O'Toole, GA;Kolter, R
通讯作者：
Kolter, R
影响因子：
3.2
作者：
Thormann, KM;Duttler, S;Spormann, AM
通讯作者：
Spormann, AM