Identification of Alkaloids in Stephania hainanensis by Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry
Identification of Alkaloids in Stephania hainanensis by Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry
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DOI：
10.1002/pca.2618
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发表时间：
2016-05-01
影响因子：
3.3
通讯作者：
Huang, Jianming
中科院分区：
文献类型：
--
作者：
He, Jiayong;Liu, Ying;Huang, Jianming
前言--千金藤属植物可产生多种生物活性生物碱。海南千金娘是一种含有有效生物碱的药用植物。目的-采用LC-QTOF-MS/MS联用技术对海南千金藤中的生物碱进行结构鉴定。方法：建立了海南千金生物碱的LC-QTOF-MS/MS方法。色谱柱采用梯度洗脱的苯柱，正电离模式下采用电喷雾电离(ESI)界面获得串联质谱图。结果在一次LC中共鉴定出37个四氢原黄连素、四氢原黄连素、阿朴吗啡、异丙酚、苄基异喹啉或双苄基异喹啉类生物碱。其中27种生物碱，包括苄基异喹啉类生物碱，以前从未在海南千金藻中报道过。提出了不同类型生物碱可能的裂解途径。结论：LC-QTOF-MS/MS方法能够对海南千金藤中的多种生物碱进行定性和定性鉴别。所得结果可能有助于了解海南链霉菌的生物活性和评价该植物的质量。版权所有(C)2016 John Wiley&Sons，Ltd.
Introduction - Plants in the genus Stephania can produce diverse bioactive alkaloids. Stephania hainanensis is a medicinal plant that contains effective alkaloids. However, only 10 alkaloids have been reported in this species.Objective - To characterise the alkaloids in Stephania hainanensis using liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (LC-QTOF-MS/MS).Methods - An LC-QTOF-MS/MS method was developed for structural characterisation of the alkaloids in Stephania hainanensis. The chromatographic separation was performed on a phenyl column with gradient elution, and the tandem mass spectra were obtained by using an electrospray ionisation (ESI) interface in positive ionisation mode. Compound identification was based on the exact masses, fragmentation pathways, retention behaviours and related botanical biogenesis.Results - A total of 37 tetrahydroprotoberberine-, quaternary protoberberine-, aporphine-, proaporphine-, benzylisoquinoline- or bisbenzylisoquinoline-type alkaloids were identified or tentatively identified in a single LC run. Twenty-seven of these alkaloids, including the benzylisoquinoline-type of alkaloids, have not been previously reported in Stephania hainanensis. The possible fragmentation pathways of different types of alkaloids were proposed. Besides the general fragmentations, the characteristic losses of CH3N=CH2 were observed for the benzylisoquinoline and aporphine alkaloids with two methyl groups on the nitrogen.Conclusion - The LC-QTOF-MS/MS method enabled profiling and rational, but tentative, identification of diverse alkaloids in Stephania hainanensis. The results obtained may be helpful for understanding the bioactivity of S. hainanensis and evaluating the quality of this plant. Copyright (C) 2016 John Wiley & Sons, Ltd.