Linkage of biochemical responses to population-level effects:: A case study with vitellogenin in the fathead minnow (Pimephales promelas)
Linkage of biochemical responses to population-level effects:: A case study with vitellogenin in the fathead minnow (Pimephales promelas)
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DOI：
10.1897/06-318r.1
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发表时间：
2007-03-01
影响因子：
4.1
通讯作者：
Ankley, Gerald T.
中科院分区：
文献类型：
--
作者：
Miller, David H.;Jensen, Kathleen M.;Ankley, Gerald T.
在生态毒理学领域的一个挑战是在分子和生物化学水平的组织的变化，在个人和群体的不良后果的联系。在本研究中，血浆卵黄蛋白原（VTG）的浓度和繁殖力之间的预测关系，在雌性黑头呆鱼（黑头呆鱼）来自21天的实验室毒性试验与5种化学品（17 β-群勃龙，17 α-群勃龙，咪鲜胺，fenarimol，和法倔唑），抑制VTG生产通过不同的机制。由于VTG是雌性卵生动物产卵的关键，理论上，脂蛋白的变化可以作为生殖成功的指标。生殖力与VTG浓度的回归得到了一个高度显著的线性模型（生殖力= -0.042 +0.95-VTG，p < 0.01，r（2）= 0.88）。这种关系被整合到一个人口模型中的VTG浓度的变化，翻译的女性黑头呆鱼在人口增长的变化。该模型预测相对深刻的影响，鱼类的人口规模经历适度减少卵黄。例如，一个黑头呆鱼种群在承载能力暴露于化学应激源，导致VTG浓度从基线值下降25%的女性将表现出34.6%的预计减少后，两年的曝光和达到平衡的人口规模，只有30.2%的暴露前的人口。总的来说，目前的研究提供了一个例子，说明生物标志物（VTG浓度）的变化如何在个体和群体水平上定量转化为不良反应。
A challenge in the field of ecotoxicology is the linkage of alterations at molecular and biochemical levels of organization to adverse outcomes in individuals and populations. In the present study, a predictive relationship between plasma vitellogenin (VTG) concentration and fecundity in female fathead minnows (Pimephales promelas) was derived from 21-d laboratory toxicity tests with five chemicals (17 beta-trenbolone, 17 alpha-trenbolone, prochloraz, fenarimol, and fadrozole) that inhibit VTG production through different mechanisms. Because VTG is key to egg production in female oviparous animals, changes in the lipoprotein could, theoretically, serve as an indicator of reproductive success. Regression of fecundity versus VTG concentration from the various studies yielded a highly significant linear model (fecundity = -0.042 + 0.95-VTG, p < 0.01, r(2) = 0.88). This relationship was integrated into a population model to translate changes in VTG concentrations of female fathead minnows to alterations in population growth. The model predicted relatively profound effects on population size of fish experiencing moderate decreases in vitellogenesis. For example, a fathead minnow population at a carrying capacity exposed to a chemical stressor that causes a 25% decrease in VTG concentration in females from baseline values would exhibit a 34.6% projected decrease in size after two years of exposure and reach an equilibrium population size that was only 30.2% of the preexposed population. Overall, the current study provides an example of how changes in a biomarker (VTG concentration) can be quantitatively translated into adverse effects at the individual and population levels.