NEUROPROTECTIVE AND NEUROREGENERATIVE EFFECTS OF LOW-INTENSITY AEROBIC EXERCISE ON SCIATIC NERVE CRUSH INJURY IN MICE
NEUROPROTECTIVE AND NEUROREGENERATIVE EFFECTS OF LOW-INTENSITY AEROBIC EXERCISE ON SCIATIC NERVE CRUSH INJURY IN MICE
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DOI：
10.1016/j.neuroscience.2011.07.075
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发表时间：
2011-10-27
期刊：
影响因子：
3.3
通讯作者：
Santos, A. R. S.
中科院分区：
文献类型：
--
作者：
Bobinski, F.;Martins, D. F.;Santos, A. R. S.
在这里，我们建立了一个低强度有氧运动的程序，并比较了运动术前，术后，以及术前和术后方案的组合对小鼠坐骨神经挤压损伤恢复的影响，使用行为，生化和形态学分析。对成年雄性小鼠进行坐骨神经挤压。动物接受术前（2周）、术后（2周）和术前-术后（4周）联合训练方案。在训练期间，使用坐骨神经功能指数、坐骨神经静态指数以及机械和冷超敏反应分析来监测功能恢复。在破碎后第14天分析形态学和生化变化。所有运动组的功能恢复值均显著优于非运动组。从生物化学上讲，所有运动组坐骨神经中的白细胞介素-1 β（IL-1 β）以及脊髓中的IL-1 β和白细胞介素-6受体（IL-6 R）水平的增加都有所减少。然而，肿瘤坏死因子-α（TNF-α）水平仅在术后组和联合运动方案中下降。在形态学分析中，组合运动受试者呈现纤维和轴突直径增加，髓鞘化程度和有髓纤维数量增加。目前的研究表明，术前和术后锻炼的坐骨神经再生的功能和形态的价值，显着优于术前或术后协议。这项实验研究表明，体育锻炼可以恢复运动和神经功能，在很大程度上使用预防和治疗的方法进行。(C)2011年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Here, we established a program of low-intensity aerobic exercise and compared the effects of exercise preoperative, postoperative, and a combination of both pre- and postoperative protocols on recovery from sciatic nerve crush injury in mice using behavioral, biochemical, and morphological assays. Sciatic nerve crush was performed in adult male mice. The animals were submitted to preoperative (for 2 weeks), postoperative (for 2 weeks), and a combination of preoperative-postoperative (for 4 weeks) training protocols. During the training period, functional recovery was monitored using the Sciatic Functional Index, the Sciatic Static Index, and mechanical and cold hypersensitivity analyses. Morphological and biochemical alterations were analyzed on the 14th day post-crushing. The functional recovery values of all of the exercised groups were significantly better than the nonexercised group. Biochemically, all of the exercise groups showed a reduction in the increase of interleukin-1 beta (IL-1 beta) in the sciatic nerve and in the IL-1 beta and interleukin-6 receptor (IL-6R) levels in the spinal cord. However, the levels of tumor necrosis factor alpha (TNF-alpha) decreased only in the postoperative group and in the combination exercise protocols. In the morphological analysis, the combination exercise subjects presented an increase in fiber and axon diameter, in the myelination degree and in the number of myelinated fibers. The present study showed that pre- and postoperative exercise achieved values for functional and morphological sciatic nerve regeneration that were significantly better than either the preoperative or postoperative protocols. This experimental study suggests that physical exercise can restore motor and nerve function to a substantial degree when performed using a prophylactic and therapeutic approach. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.