Solid-phase microextraction (SPME) with stable isotope calibration for measuring bioavailability of hydrophobic organic contaminants.
Solid-phase microextraction (SPME) with stable isotope calibration for measuring bioavailability of hydrophobic organic contaminants.
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DOI：
10.1021/es4022987
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发表时间：
2013-09-03
影响因子：
11.4
通讯作者：
Gan, Jay
中科院分区：
文献类型：
--
作者：
Cui, Xinyi;Bao, Lianjun;Gan, Jay
固相微萃取（SPME）是一种仿生工具，非常适合于测量沉积物和土壤基质中疏水有机化合物（hoc）的生物利用度。然而，传统的SPME取样需要在纤维和样品基质之间达到平衡，这可能需要数周或数月的时间，这极大地限制了其适用性。在这项研究中，我们探索了用稳定同位素标记类似物（SI-SPME）预加载聚二甲基硅氧烷纤维，以避免长采样时间的需要，并使用一系列沉积物和条件评估了SI-SPME与传统平衡SPME （Eq-SPME）的性能。稳定同位素标记类似物的解吸和对PCB-52、PCB-153、联苯菊酯和顺式氯菊酯的吸附均呈各向同性，验证了SI-SPME的假设。以丙酮-水（1:4,v/v）为载体，实现了高重复性的预压。与Eq-SPME需要数周甚至数月的时间相比，SI-SPME可以在搅拌条件下的1天内可靠地估算出平衡状态下的纤维浓度（Cf），或者在加钉沉积物的静态条件下的20天内可靠地估算出纤维浓度。SI-SPME预测的Cf值与Eq-SPME预测的Cf值在统计学上相同。SI-SPME法进一步成功应用于被PCB 52、PCB 153和联苯菊酯污染的野外沉积物中。如今，稳定同位素标记标准物和质谱法的可用性越来越高，使得SI-SPME非常可行，允许在非平衡条件下使用SPME，采样时间更短或灵活。
Solid-phase microextraction (SPME) is a biomimetic tool ideally suited for measuring bioavailability of hydrophobic organic compounds (HOCs) in sediment and soil matrices. However, conventional SPME sampling requires the attainment of equilibrium between the fiber and sample matrix, which may take weeks or months, greatly limiting its applicability. In this study, we explored the preloading of polydimethylsiloxane fiber with stable isotope labeled analogs (SI-SPME) to circumvent the need for long sampling time, and evaluated the performance of SI-SPME against the conventional equilibrium SPME (Eq-SPME) using a range of sediments and conditions. Desorption of stable isotope-labeled analogs and absorption of PCB-52, PCB-153, bifenthrin and cis-permethrin were isotropic, validating the assumption for SI-SPME. Highly reproducible preloading was achieved using acetone-water (1:4, v/v) as the carrier. Compared to Eq-SPME that required weeks or even months, the fiber concentrations (Cf) under equilibrium could be reliably estimated by SI-SPME in 1 d under agitated conditions or 20 d under static conditions in spiked sediments. The Cf values predicted by SI-SPME were statistically identical to those determined by Eq-SPME. The SI-SPME method was further applied successfully to field sediments contaminated with PCB 52, PCB 153, and bifenthrin. The increasing availability of stable isotope labeled standards and mass spectrometry nowadays makes SI-SPME highly feasible, allowing the use of SPME under non-equilibrium conditions with much shorter or flexible sampling time.
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影响因子：
11.4
作者：
Booij, Kees;Smedes, Foppe;Honkoop, Pieter J. C.
通讯作者：
Honkoop, Pieter J. C.
影响因子：
8.8
作者：
Booij, K;Smedes, F;van Weerlee, EM
通讯作者：
van Weerlee, EM
影响因子：
11.4
作者：
Reid, BJ;Stokes, JD;Semple, KT
通讯作者：
Semple, KT
影响因子：
4.5
作者：
Cui, Xinyi;Hunter, Wesley;Gan, Jay
通讯作者：
Gan, Jay
影响因子：
11.4
作者：
Jonker, Michiel T. O.;van der Heijden, Stephan A.;Hawthorne, Steven B.
通讯作者：
Hawthorne, Steven B.