Development of a nested PCR assay for detection of feline infectious peritonitis virus in clinical specimens
Development of a nested PCR assay for detection of feline infectious peritonitis virus in clinical specimens
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DOI：
10.1128/jcm.35.3.673-675.1997
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发表时间：
1997-03-01
影响因子：
9.4
通讯作者：
Colucci, G
中科院分区：
文献类型：
--
作者：
Gamble, DA;Lobbiani, A;Colucci, G
开发了一种基于巢式PCR（nPCR）检测的猫传染性腹膜炎病毒（FIPV）感染诊断检测方法，并使用FIPV、猫肠道冠状病毒（FECV）、犬冠状病毒（CCV）和传染性胃肠炎病毒（TGEV）以及患有渗出性猫传染性腹膜炎（FIP）的猫的临床液体样本进行了检测。该检测试剂的靶序列位于peplomer蛋白E2基因的S1区。FIPV疫苗株和两种野生型FIPV毒株检测结果为阳性，但FECV、TGEV和CCV检测结果为阴性。对12只具有渗出性FIP临床证据的猫和11只患有与渗出相关但归因于其他原因的疾病的猫进行了初步检测。12只渗出性FIP猫中11只阳性，1只阴性; 11只其他原因引起的FIP猫中10只阴性，1只阳性。根据临床实验室和组织病理学标准，该检测方法的初步敏感性和特异性分别为91.6%和94%。
A diagnostic test for feline infectious peritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FIPV, feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV) and clinical fluid samples from cats with effusive feline infectious peritonitis (FIP). The target sequence for the assay is in the S1 region of the peplomer protein E2 gene, A vaccine strain of FIPV and two wild-type FIPV strains tested positive, but FECV, TGEV, and CCV tested negative, Preliminary tests with 12 cats with clinical evidence of effusive FIP and 11 cats with an illness associated with effusions, but attributed to other causes, were performed. Eleven of the 12 cats with effusive FIP tested positive, while 1 was negative, Ten of the 11 cats ill from other causes tested negative, while 1 was positive. On the basis of clinical laboratory and histopathologic criteria, the preliminary sensitivity and specificity of the assay were 91.6 and 94%, respectively.