Colocalization between caveolin isoforms in the intestinal smooth muscle and interstitial cells of Cajal of the Cav1+/+ and Cav1-/- mouse
Colocalization between caveolin isoforms in the intestinal smooth muscle and interstitial cells of Cajal of the Cav1+/+ and Cav1-/- mouse
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DOI：
10.1007/s00418-005-0128-3
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发表时间：
2006-07-01
影响因子：
2.3
通讯作者：
Daniel, Edwin E.
中科院分区：
文献类型：
--
作者：
Cho, Woo Jung;Daniel, Edwin E.
共聚焦显微镜下观察Caveolin-1、Caveolin-2和Caveolin-3在Cav 1（+/+）和Cav 1（-/-）小鼠小肠平滑肌细胞（SMCs）和Cajal间质细胞（ICC）中的定位和共定位。通过LSM 5分割肠区域[内环肌（icm）、外环肌（ocm）、肌间神经丛区域（mp）和纵肌（lm）]，并通过ImageJ分析以显示共定位的皮尔逊相关性（r（p））和重叠系数（r）。在Cav 1（+/+）的肠道中，caveolin-1（cave 1）与caveolin-2（cave 2）和caveolin-3（cave 3）共定位。Cav 2也与Cav 3良好共定位。在Cav 1（-/-）的肠中，cav 1和cav 2在所有图像中均缺失，但cav 3在ocm中表达减少。Cav 1（+/+）细胞中存在cav 1细胞类型的小窝，Cav 1（-/-）细胞中存在cav 3细胞类型的小窝。C-kit在Cav 1（+/+）和Cav 1（-/-）肠内均存在于深肌丛（ICC-MP）和肌间神经丛（ICC-MP），并与Cav 1和Cav 2共定位于Cav 1（+/+）肠内。Cav 3在Cav 1（+/+）和Cav 1（-/-）肠道的ICC-MP和ICC-MP中不存在/存在低免疫反应性。结论：cav 1对cav 2在小肠SMC和ICC中的表达是必需的，并对cav 3的表达起促进作用，但对cav 3的表达不是必需的。
Confocal microscopic images were obtained from the immunohistochemical sections of jejeunum to determine the localization/colocalization between caveolin-1, caveolin-2 and caveolin-3 in intestinal smooth muscle cells (SMCs) and interstitial cells of Cajal (ICC) of Cav1(+/+) and Cav1(-/-) mouse. Intestinal regions were segmented [inner circular muscle (icm), outer circular muscle (ocm), myenteric plexus region (mp), and longitudinal muscle (lm)] by LSM 5 and analyzed by ImageJ to show Pearson's correlation (r (p)) and overlap coefficient (r) of colocalization. In the intestine of Cav1(+/+), caveolin-1 (cav1) was colocalized with caveolin-2 (cav2) and caveolin-3 (cav3). Cav2 also was well colocalized with cav3. In the intestine of Cav1(-/-), cav1 and cav2 were absent in all images, but reduced cav3 was expressed in ocm. Caveolae were present in cell types with cav1 in Cav1(+/+), and present with cav3 in ocm of Cav1(-/-). C-kit occurred in deep muscular plexus (ICC-DMP) and myenteric plexus (ICC-MP), in both Cav1(+/+) and Cav1(-/-), and colocalized with cav1 and cav2 in the intestine of Cav1(+/+). Cav3 was absent/present at low immunoreactivity in ICC-DMP and ICC-MP of the intestines of Cav1(+/+) and Cav1(-/-). To conclude, cav1 is necessary for the expression of cav2 in SMC and ICC of intestine and facilitates, but is not necessary for the expression of cav3.