Gram-positive bacteria are a major reservoir of Class 1 antibiotic resistance integrons in poultry litter
Gram-positive bacteria are a major reservoir of Class 1 antibiotic resistance integrons in poultry litter
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DOI：
10.1073/pnas.0306466101
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发表时间：
2004-05-04
影响因子：
11.1
通讯作者：
Summers, AO
中科院分区：
文献类型：
--
作者：
Nandi, S;Maurer, JJ;Summers, AO
逆转抗生素多重耐药细菌的传播受到了对耐药基因自然历史、携带它们的移动的元素以及携带它们的细菌[宿主]的无知的阻碍。使用传统的培养和不依赖培养的分子技术，我们量化了商业家禽养殖场家禽粪便中的抗生素耐药性基因和称为整合子的移动的元件。出乎意料的是，家禽垃圾中1类整合子的主要储存库不是它们先前鉴定的宿主，革兰氏阴性肠杆菌科，如大肠杆菌。相反，整合子和相关的抗性基因在几个属的革兰氏阳性细菌中大量存在，这些革兰氏阳性细菌构成了>85%的凋落物群落，而肠杆菌科包括
Reversing the spread of antibiotic multiresistant bacteria is hampered by ignorance of the natural history of resistance genes, the mobile elements carrying them, and the bacteria[ hosts harboring them. Using traditional cultivation and cultivation-independent molecular techniques, we quantified antibiotic resistance genes and mobile elements called integrons in poultry house litter from commercial poultry farms. Unexpectedly, the major reservoir for Class 1 integrons in poultry litter is not their previously identified hosts, Gram-negative Enterobacteriaceae such as Escherichia coli. Rather, integrons and associated resistance genes abound in several genera of Gram-positive bacteria that constitute >85% of the litter community compared with Enterobacteriaceae that comprise